Poonam Mehta

2 Questions 3 Answers 0 Followers

Questions related from Poonam Mehta

In case of low concentration, can NGS libraries be re-amplified?

I have prepared small RNA libraries from 1ng of total RNA using smRNA seq kit of Takara. Bioanalyzer profile before size selection showed 3-5nM library of desired size; however after size...

31 August 2022 2,034 0 View

How many PGCs can be isolated from gonads of advanced embryonic stages such as 14.5dpc or 16.5dpc resulting from B6;CBA-Tg Pou5f1-EGFP animal mating?

We have B6;CBA-Tg(Pou5f1-EGFP) animal model from Jackson. I am trying to collect primordial germ cells from the different embryonic stages. Acc. to available literature, there are 25000 PGCs in...

27 March 2022 8,018 8 View