How could I stop the autoproteolysis process?

07 August 2017 1,191 6 View

How can I remove DNAk from my protein solution?

31 December 2016 2,542 3 View

A rare purification How could I obtain the monomer or one of the oligomers?

31 December 2016 6,336 2 View

What can i do so my protein will be finished the folding?

Hi all. I have a pure protein but in SDS-PAGE gel there is DNAk in the solution too ( It is together with my protein). In Superose6 column I have 3 proximate peaks which I cant separated. I think...

31 December 2016 3,167 8 View

Protocol for E.coli expression/induction with a Heat shock step?

Hi all. I want to high the amount of the Heat shock chaperones concentration. I read one option is to do a Heat shock step before the protein induction. Anyone know How could I do? Thank you.

31 December 2016 6,985 2 View

What Could I do for improve on the protein expression for end the fold of my protein ?

31 December 2016 8,390 5 View

Could My protein be affected with the sonication method?

11 December 2016 4,556 12 View

Why after centrifugation at 13,2RPM during 2 hours does my protein broken in the linear monormer?

Hello. My name is David Vizarraga and I am a PhD student. I am trying to purify a protein but I always have a oligomer mix in the solution and I can't crystallize it (because I need a pure...

10 November 2016 5,136 3 View

Which is the perfect buffer for my protein?

Hi all I want to purify a protein (Pi:4,0) and I am use a Typical buffer (50mM Tris pH: 7,4, 200mM NaCl and 10mM glucose ( decreased the aggregates and It is better for the crystallization than...

10 November 2016 9,609 1 View

My protein forms aggregates but I couldnt use additives like glucose or glycerol because It forms oligomers What could I do in my purification step?

10 November 2016 2,739 9 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

What solvent can I used for Paal Knorr reaction between 6-amino hexanoic acid and 2.5 hexanedione?

I am try to make the Paal Knorr reaction between 2.5 hexnedione and 6-amino hexanoic acid, my problem is type of solvent to use, because 6-hexaminohexanoic acid is soluble in water but I am not...

02 March 2021 4,443 2 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

How to compare different concentrations of nanoparticles for characterization from ICP-MS?

I took measurements of different concentrations of 15 nm and 5 nm Au nano particle solutions. I'm trying to determine which concentration I measured gives the best reading overall. I also...

02 March 2021 7,626 1 View

What are the new 2D nanomaterials were used for Silver nanoparticles stabilization?

In the stabilization of AgNPs are vital in current research interest. However, some of the 2D nano-materials like graphene oxide, MoS2, etc... previously used to stabilize it. But still they shown...

01 March 2021 2,207 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Why am i not getting solid after reacting APTES with salicylaldehyde?

The reaction of APTES with salicylaldehyde in ethanol leads to the formation of imine compound yellow solid which is mention in literature, but I am not getting yellow solid I don't know why? when...

28 February 2021 2,955 2 View

I want to performe MALDI-TOF MS for bacterial identification, I would appreciate it if anyone can tell me the matrix solution preparation protocol ?

based on what I've studied on multiple papers, for peptide and protein identification Alpha-cyano-4-hydroxycinnamic acid (CHCA) is an appropriate matrix to use, I also know that the CHCA powder...

26 February 2021 6,799 3 View

Why there are two peaks while using the mie plot of Silver nanoparticle?

Hi, I am trying to learn the theoretical modelling of silver nanoparticle using the Mie scattering theory. After that, I discovered a software named Mieplot developed by Philip Laven, where it...

25 February 2021 7,493 6 View

How to best wash a 96-well plate containing microplastic?

I am planning my protocol for my dissertation for an experiment quantifying bacterial (E. coli) biofilm formation on micro-plastic (5mm discs of polyethylene bags) by conducting a crystal violet...

23 February 2021 7,868 2 View