What do you think is the associated disease?

More Shaira Lagas's questions See All

Can anyone recommend me a well organized playlist or a full course on any platform where I can learn A-Z of PyPSA(Python for power System Analysis)?

Python for Power System Analysis (PyPSA) is a free software toolbox for simulating and optimizing modern electrical power systems over multiple periods. PyPSA includes models for conventional...

26 February 2023 1,363 4 View

What is the optimum Wolbachia population that can significantly affect dengue cases in densely populated provinces in the country?

I am working on a paper that looks into the dynamics of the spread of Wolbachia and its potential impact on dengue transmission, particularly in the Philippines.

29 October 2020 7,038 3 View

Which release method is most efficient in driving the Wolbachia population to its optimum level?

I am working on a paper that looks into the dynamics of the spread of Wolbachia and its potential impact on dengue transmission, particularly in the Philippines. Is there a most efficient existing...

29 October 2020 3,802 0 View

What are the BCG vaccine effects on TB skin test?

If one have been vaccinated with BCG vaccine for Mycobacterium tuberculosis, will they test positive for TB skin test even though they are not infected?

28 April 2020 5,691 2 View

What might be the root cause of the traffic congestion?

Impact towards stakeholders such as businesses due to bottlenecks.

07 November 2019 3,523 9 View

What are the characteristics of a good oil quality? What are the factors that may affect the degradation of its quality?

Oils, particularly palm and coco oils, are essentially regarded in terms of quality based on its Free Fatty Acid (FFA) Value, Iodine Number, and Peroxides Value. What is the relationship between...

01 February 2019 3,883 4 View

How to interpret DOS graphs from DFT calculations?

Hi, I have some specific questions regarding the interpretation of DOS plots from DFT softwares (e.g. VASP). 1. What does it mean when the peaks broadened after material alterations (e.g. addition...

03 July 2017 7,843 6 View

Can we use VASP for DOS calculation of isolated molecule in vacuum?

Hi, I am studying how to use VASP to obtain DOS for several systems. I'm trying it on H2 molecule and I get really weird results. All the states are way above Fermi level. I am thinking that DOS...

25 June 2017 3,783 5 View

How to perform Bader Charge Analysis steps from VASP output?

Hi, I just want to verify if these are the correct steps to Bader Charge Analysis: Steps: 1. Relax the system to a converged geometry. 2. Using the relaxed geometry, perform static calculation...

19 January 2017 494 15 View

Any idea how long does it take to rear spiders?

From the breeding period of spiders up to the full growth of the offsprings, do you have any idea what would be the approximate duration of the entire process? Specifically, those spiders...

18 October 2016 2,460 4 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

How to use ImageJ for fluorescence quantification of platelet adhesion and spreading?

I am a beginner with ImageJ and would like to know how to quantify fluorescence images of platelets taken with a fluorescence microscope at 40x and 100x magnification. If you could provide...

11 July 2024 1,464 7 View

Is there a way to calculate cell density and thus cell count from confluency for RPE-1 cells?

I am growing cells in a 24 well plate. I do not have access to a microscope with a camara, so I have to calculate the number of cells in the wells manually. I can get the confluency pretty easily,...

04 July 2024 9,622 0 View

Any tips for cell counting in 96-well plate?

Dear colleagues, I am currently using HCT-116 adherent cells for cell culture in 96-well plates and have some questions regarding cell counting. I have tried various methods and found the most...

22 June 2024 1,124 5 View

How do you calculate sample size for a clinical trial when you only have cross-sectional data of the outcome parameter?

Hello, I have cross-sectional data on immune cell density in a disease group. Compared to controls, the disease group shows an increase in immune cell density. Now, I want to run a clinical...

17 June 2024 6,257 3 View

Pseudomonas aeruginosa colonies are too transparent: any tips on counting?

Hi everyone. I need to do a lot of colony counting tomorrow and when I was doing it today I’ve noticed that most of the colonies are too transparent - I have to shine bright light on them all the...

12 June 2024 711 3 View

Optimal conditions for lentiviral transduction of B-ALL cells (I'm using Nalm6 cells)?

Dear Esteemed Researchers, I am currently waist-deep into the literature to determine the optimal cell density and transduction volume for lentiviral transduction conducted in 24- and 48-well...

10 June 2024 4,167 0 View

Can you explain the potential toxicity and overdosemanagement of commonly used antiplatelet drugs?

Antiplatelet Therapy: Mechanisms, Indications, Administration Can you explain the potential toxicity and overdosemanagement of commonly used antiplatelet drugs?

05 June 2024 6,447 1 View

Is there a good protocol for FFPE fixation of defrozen PBMCs?

Hi all, I have been scouring the internet for a protocol for defrozen PBMC fixation and embedding. Currently I am defreezing PBMCs with an in-house protocol which gives me ~95% viability. After...

29 May 2024 7,463 2 View

I want to standardize the human platelet antigen primers for HPA genotyping. What protocol I should follow?

Usually, I use cDNA and perform gradient PCR for primer standardization. What kind of sample cDNA do I have to use in this case and what steps do I need to consider for standardization?

26 May 2024 8,124 0 View

Fahad Alshehri

What is the blood group of the patient? Is it O grouping?

Emad Taherabadi

Hi Shaira,

Based on the clinical findings (the onset of mucocutaneous bleeding in childhood) and the decrease in the platelet count and the non-response of the aggregation test to ristocetin, it seems that the patient is suffering from Bernard–Soulier syndrome. Examining PBS for the presence of giant platelets and measuring the surface expression of GPIbα is helpful.