Which alginate is best for making gel for wound dressing application?

We are looking into utilizing alginate as a gel coating on an HDPE substrate for drug delivery. I would like to ask the community about their experience with alginate and which commercial sources...

15 February 2021 4,319 4 View

What is the optimum Wolbachia population that can significantly affect dengue cases in densely populated provinces in the country?

I am working on a paper that looks into the dynamics of the spread of Wolbachia and its potential impact on dengue transmission, particularly in the Philippines.

29 October 2020 6,723 3 View

Has anyone tried performing a transient transfection on a cell line that has been stably infected with a lentivirus?

Hello, I was having difficulty in infecting a cell with a gene of interest. Say, for instance, I was able to introduce a CMV on the cell, but I was not successful in introducing my target gene. I...

25 September 2019 2,412 3 View

Mouse Cell Line STR Profiles?

Does anyone have STR profiles of mouse cell lines from the following strains: C57BL/6 129 FVBN BALB/c Swiss black Which company did you use to authenticate the cells?

07 April 2019 4,519 2 View

Can 2 way ANOVA be used in nonexperimantel research?

We will be studying about the role of spirituality and gender ( 2 IVs) on coping effectiveness (DV) and we shall be using standardized scales in measuring. Can 2 way ANOVA be used in our analysis?...

09 November 2017 8,917 4 View

How to interpret DOS graphs from DFT calculations?

Hi, I have some specific questions regarding the interpretation of DOS plots from DFT softwares (e.g. VASP). 1. What does it mean when the peaks broadened after material alterations (e.g. addition...

03 July 2017 7,319 6 View

Any information or studies about Wild Sampaguita (Jasminum bifarium) ?

My study for my undergraduate thesis is Phytochemical Evaluation, Antibacterial Activity and Angiogenic Property of Wild Sampaguita (Jasminum bifarium). I am using leaves for my study and I need...

25 March 2017 3,487 3 View

Does anyone have troubleshooting experience for low on-target exome enrichment (Nextera)?

I have prepared 4 libraries (Nextera exome 37mb), first with 12 samples and prepared 3 months before the next 3 (of 24 samples). The first had low enrichment (40% of reads on-target), the second...

30 June 2015 3,324 1 View

How do you make VASP calculations faster?

I saw that there are many ways to make VASP calculations faster. I have some questions regarding these methods. (1)Set ALGO=F. Basically, this uses Blocked Davidson algorithm in the first five...

19 January 2015 3,783 14 View

How common is five-fold symmetry among plants and animals?

06 July 2014 6,460 3 View

What are the most reliable approaches to extract key continuous frames from a video, with complete automation?

I have an input video, of skin (from a microscope that means it is magnified such that blood capillaries are visible). The idea is to extract 15 sec of continuous frames from a video of 1 min,...

02 March 2021 2,735 2 View

What is the most suitable server for predicting protein structures in bacteria?

I have used the i-Tasser several times, however it has been unavailable for several days. I tried the swiss-model, but the output was not very pleasant due to the model used. Is there any other...

28 February 2021 4,521 3 View

How can we simulate an hyperelastic-viscoelastic behavor with Ansys APDL?

I am working on modeling and simulation of biomecanical material behaviour, I have succeded on simulating skin using anisotropic hyperelastic material, on APDL and in our lab's finite element...

28 February 2021 552 3 View

What is the most common number of epitopes in an multi-epitope Vaccine?

hi everyone, I'm interested in the field of vaccine design specifically multi-epitope cancer vaccines , I want to know if there is a minimum and maximum number of epitopes we could use in a...

27 February 2021 3,882 3 View

What is the best surface to culture cells isolated from a skin biopsy?

Hello, recently I am having some difficulties with maintaining the cell culture of primary cells isolated from a skin biopsy (normal and malignant). The coating of culture plates with Matrigel (a...

26 February 2021 6,151 2 View

Can ImageJ software be used to quantify collagen from Masson's-Trichrome stained images?

I am after quantifying the blue stained collagen in a Masson's-Trichrome stained skin section from an immune deficient mice and compared the same with its WT control. I am curious to know can...

17 February 2021 384 3 View

Cells not growing in large scale protein expression?

I am using KRX cell. I have grown my overnight cultures at 37 degrees. When I inoculate 1L TB media, my cells do not grow. I have also tried using 25 degree incubation of overnight cultures and...

10 February 2021 3,871 3 View

Differences between sheep blood with Alsever solution vs citrated/defibrinated sheep blood in microbiological media?

I am aware of a study that has compared the use of citrated and defibrinated sheep's blood in microbiological media (Hair Sheep Blood, Citrated or Defibrinated, Fulfills All Req... ), but can...

10 February 2021 5,927 1 View

Can anybody provide me sequences of SPF10 primers?

03 February 2021 1,053 2 View

How do you annotate figures to show significance?

30 January 2021 1,008 3 View