Linear vs Supercoiled vs Nicked DNA on agarose?

I am pretty new to molecular biology techniques and just when I think I have it all figured out, something stumps me. This is how this gel (1.5% agarose) is loaded: 1. GeneRuler ready to use cat#...

06 October 2020 7,523 4 View

Has anyone reported on carbon storage potential in common trees and shrubs in prairie forests?

Has anyone reported on carbon storage potential in common trees and shrubs in prairie forests? I am looking for carbon storage potential ie carbon biomass for a number of native trees and shrubs...

08 July 2020 5,236 2 View

Issues with Image J Thresholding?

I am wondering if anyone has had issues with their image J thresholding? I am trying to perform colour deconvolution, but when I convert the image to binary, I am unable adjust the threshold to...

01 April 2020 3,812 3 View

Where can I get survey data to compare the US to subSaharan Africa, China on the Impact of Climate Change?

Preparing a research paper. Other countries/geographies could be substituted

12 March 2020 195 4 View

Quantifying ADC values from a ROI?

I currently have both DWI and ADC sequences however I want to calculate the mean ADC value for a defined ROI (ischemic stroke lesion). I currently use ITK-SNAP, Image J and Horos for viewing...

03 March 2020 9,749 4 View

Which software is good for analysis of band size in gel image? How can we analyze the curve band image?

Currently, i am thinking about GelAnalyzer software. Please help me with the analysis and band scoring. I have used 100 bp ladder.

17 February 2020 7,762 5 View

How do I align a sequence set to one reference sequence?

I am currently trying to find homologues of a protein I am working with, but BLAST has been giving me nothing useable. I have now found a dataset of 1500 protein sequences of potential candidates...

26 June 2019 5,596 2 View

I need an analytical balance that weighs organic samples at 0.0001 g for stable isotope analysis. What models are reliable and cost-effective?

For weighing stable isotope samples

18 October 2018 687 4 View

Assuming you are teaching a class and a student ask you a question you cannot readily answer. What would you do?

When you find yourself in this situation, what would you do intelligently so your students don't believe you can't answer?

02 March 2018 818 62 View

How can drug abuse be mitigated among students?

There is this canker of students abusing drugs in school. How can this be controlled?

01 March 2018 8,003 3 View

error in running a model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

error in modelling model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

MGL tools

20 June 2021 0 0 View

i have some problem with chtMultiRegionSimpleFoam

--> FOAM FATAL ERROR: Maximum number of iterations exceeded From function Foam::scalar Foam::species::thermo::T(Foam::scalar, Foam::scalar, Foam::scalar, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar) const) const [with Thermo = Foam::hConstThermo >; Type = Foam::sensibleEnthalpy; Foam::scalar = double; Foam::species::thermo = Foam::species::thermo >, Foam::sensibleEnthalpy>] in file /home/ubuntu/OpenFOAM/OpenFOAM-4.1/src/thermophysicalModels/specie/lnInclude/thermoI.H at line 66. FOAM aborting #0 Foam::error::printStack(Foam::Ostream&) at ??:? #1 Foam::error::abort() at ??:? #2 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::calculate() at ??:? #3 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::correct() at ??:? #4 ? at ??:? #5 __libc_start_main in "/lib/x86_64-linux-gnu/libc.so.6" #6 ? at ??:?

24 March 2021 0 0 View

Guidelines for survey (question type) analysis?

Hi. Please tell me what guidelines should i need to follow for questionaries' type research work in India. It is not hospital based work, we are conduction basic institutional based qualitative...

03 March 2021 2,037 3 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

After a reference from Int J Body Compos Res, can any body help?

Hi, I am after the reference below, my library says it cannot obtain a copy either locally or internationally, any help appreciated! Chris Wang ZM, Heshka S, Wielopolski L, Pi-Sunyer FX, Pierson...

03 March 2021 6,193 1 View

What is the production process of CaFe ferroalloys?

I looking for ferro calcium production process

03 March 2021 1,755 4 View

Resistor definition in Piezoelectric bimorph with ANSYS workbench

Hello, good day! I am trying to simulate similar bimorph piezoelectric harvester with below commands. However, the output power doesn't change with resistor value. Please help to find the reason. ET,10,CIRCU94,0 !SET UP RESISTOR R,1,100000 !RESISTANCE VALUE TYPE,10 !SET ELEMENT TYPE E,1,2 !CREATE RESISTOR BETWEEN NODES 1 AND 2 Thanks in advance Regards,

02 March 2021 0 0 View

How to access MSCI KLD Stats and Sustainalytics?

Hello all, I'm currently working on my undergraduate thesis and I'm having difficulties trying to access KLD Stats and Sustainalytics because my campus Airlangga University and apparently other...

02 March 2021 8,525 1 View