I've been using 1:10 to 1:100 bleach solution, but my PI has concerns of the corrosive nature/safety of bleach. I've been trying to find an SOP for cleaning lab floors; does anyone have suggestions for disinfectants and SOP for cleaning lab floors?
Bleach os not a good disinfectant for the floor because is fast inactivated for organic load.
You should use a compound that contains a surfactant or detergent and a desinfectant (Quats, IPA,...). Contact cleanrooms solutions vendors yo find the best opción (Diversey)
To make your bench should be clean, UV should be recommend better than bleach solution for inside of your bench and outsides.
UV and radiation is most powerful procedure to make the important area clean.
And if your team can not take the good results because of contaminations, your leader should check who visit to the clean bench room .
Sorry Masaru: UV is not a recommended system for dissinfection. I has a low penetration power, also don´t works if has a shadow´s zones, needs a lot of time to achieve lethal dosis for most of the contaminants and let the residues of killed pathoegen in the surface
Masaru Kunimoto We have a good protocol for keeping our bench clean already, with UV and 70% ethanol. My question is related to the lab floor.
Javier Garcia Palomo I'm currently alternating mopping between using Quats and diluted bleach. Do you think this would be okay?
Thank you Javier, I understand your opinion and we have to discuss to more detail of clean up the operation area. Talking about clean bench for disinfection, I think that our technique is less than 19 century researcher because they had done the historical admired results with poor anti-bactria and anti-fungaus agents. For the B1,2 study, first I recommend that clear up the bench exactly with 70% alcohol with ventilation, and move the instrument to reduce a shadow´s zones as possible as you can. And UV for outsides of bench room is to reduce the infection of air pollutions.
In my research life, I have never heard such experiences that infection in cultured cell line is caused with the pathogen from the floor of a laboratory with semi-clean room with 24 hour UV without operation terms.
Sorry, I have no idea to answer this question, any more.
Masaru Kunimoto Thank you! We just didn't know if there was a preferred method that other labs utilize. We keep the room clean otherwise, so I suppose either Quats or diluted bleach should be sufficient!
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