Alternative qPCR data analyser to ABI 7500 software?

Hi, I have problems with installing ABI 7500 Software on Win 8.1. Is there any alternative software to open .eds files on my OS?

11 December 2018 9,872 1 View

How many cells would be sufficient for an ELISA experiment?

I have treated HeLa cells (a p53-null cell line) in order to increases its intracellular concentration of p53. Does anyone know many cells would be sufficient for a significant change in the ELISA...

11 December 2018 9,315 4 View

SOLVED: Any one has the experience of transfecting HeLa cells with px-458 plasmid by Calcium phosphate method?

Hi I'm trying to transfect my HeLa cells with addgene pSpCas9(BB)-2A-GFP (PX458) plasmid by CaP transfection method. However, after a long time of trial and errors, my transfection efficiency is...

07 August 2018 9,212 3 View

If I increase vector:insert ratio up to 20:1, would it increase the efficiency of ligation?

in case lower ratios do not work, can use higher concentrations of insert?

31 December 2017 3,407 4 View

Waters 2707 HPLC autosampler problem?

I am using a 2707 waters HPLC device. When I try to inject a sample, it says missing plate or rack. I changed the needle and calibrated its position but I still get the same problem. I even get...

02 March 2021 1,408 1 View

No title

02 March 2021 3,060 3 View

Should we change media after1st 6hrs after plating HUVEC bought from INVITROGEN?it's not recomended by invitrogen.Will DMSO present in stock matter?

We have HUVEC bought from Invitrogen. Cat no -C-003-5c. while first plating the cells we have bought the protocol suggests not to centrifuge the cell with media to remove the DMSO. Should we...

02 March 2021 3,713 2 View

How to normalise data on OPM package from R Studio ?

Good afternoon, I recently used OmniLog from BIOLOG for my experimentations : I tested the metabolism of different strains on 2 types of plates. I have 16 strains of 3 different groups...

02 March 2021 3,584 1 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

Anyone that can help with details in phosphopeptides enrichment?

Anyone know the amount of protein before enzymatic digestion for phosphopeptides enrichment of serume sample in Ti-IMAC? Has anyone ever use Ti-IMAC(the material is from Chinese company named...

28 February 2021 7,310 3 View

Does anyone use antinuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples?

I am looking for anti-nuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples. Any suggestions highly appreciated. Thank you.

28 February 2021 6,512 3 View

Why does intensity drops as concentration increases?

Is there any reason for decrease in fluorescence emission intensity of HSA,BSA when increase the concentration ?

28 February 2021 3,653 4 View

No title

28 February 2021 9,936 2 View

Benefits of the 24 welled plate?

We have always used the 96 welled plate, the 48 welled plate but apparently there is much evidence on the 24 welled plate and above all its benefits.It states it is the same you would use on ELISA...

28 February 2021 1,149 1 View