Mohamad Pirouzfar

5 Questions 7 Answers 0 Followers

Questions related from Mohamad Pirouzfar

What could be these tree-shaped micro-organisms in my cell culture plates?

I cultured my HeLa cells in a sterile 6well cell culture plate. They grew successfully and I harvested them after a while. I left the plate capped with literally no trace of medium of FBS in it....

01 January 2019 8,465 3 View

How many cells would be sufficient for an ELISA experiment?

I have treated HeLa cells (a p53-null cell line) in order to increases its intracellular concentration of p53. Does anyone know many cells would be sufficient for a significant change in the ELISA...

12 December 2018 9,371 4 View

Alternative qPCR data analyser to ABI 7500 software?

Hi, I have problems with installing ABI 7500 Software on Win 8.1. Is there any alternative software to open .eds files on my OS?

12 December 2018 9,949 1 View

SOLVED: Any one has the experience of transfecting HeLa cells with px-458 plasmid by Calcium phosphate method?

Hi I'm trying to transfect my HeLa cells with addgene pSpCas9(BB)-2A-GFP (PX458) plasmid by CaP transfection method. However, after a long time of trial and errors, my transfection efficiency is...

08 August 2018 9,273 3 View

If I increase vector:insert ratio up to 20:1, would it increase the efficiency of ligation?

in case lower ratios do not work, can use higher concentrations of insert?

01 January 2018 3,502 4 View