I have treated HeLa cells (a p53-null cell line) in order to increases its intracellular concentration of p53. Does anyone know many cells would be sufficient for a significant change in the ELISA data?
Actually I want to treat my cells in 6-well plates (~200,000 cells per well). So how many wells I must use for each of my conditions?
I recently performed an ELISA using the supernatant from a 24 well plate. An almost confluent well for each condition should be ok (prepare triplicates)
I think it depends on the cytokines you want to detect, a 24 well plate should be enough and try dilute different folds so the concentration can fall into the standard sample curve
You can use ~250,000 cells per well in 6 well plates. For each condition, duplicate would be enough.
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