I am working on bioethanol production from terrestrial and salt tolerance yeast strain.While estimating the intracellular glucose and glycerol content I have used yeast strains grown in YPD media from 0 to 6 hours at 30 degree celcius. In order to perform lysis I have used a) water bath based sonicator set at 80 degree for 15 mins b) Probe sonicator for 180 seconds at 30 percent amplitude Since the yeast grows from 0 to 6 hrs, the intracellular glucose and glycerol level are expected to increase with time.However, I have got inconsistency in both the glucose and glycerol levels from 0 to 6 hours upon HPLC based quantitation. Please suggest any alternative method for lysis if necessary. Thanks in advance.
Hi there,
Why do you expect intracellular glucose to increase significantly during growth? Growth is based on glucose uptake and concomitent consumption therefore in exponential growth phase intracellular free glucose should remain constant as glucose consumption is constant (steady state).
- Badges
- Science topic
- Similar topics
- Physical Sciences
- Atomic, Molecular and Optical Physics
- Atoms