Binita Dev

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Questions related from Binita Dev

For laccase assay, no color change of reaction mixture was detected when ABTS is added. What might be the reason?

Presently I am working on bacterial laccase mediated delignification of lignocellulosic biomass. In regards to that, the isolated bacteria was grown in Luria Broth and the laccase assay was...

26 July 2021 8,551 0 View

How to prepare lignin basic media ?

I want to prepare bacterial media containing Kraft lignin ( dissolved in 0.1 M NaOH). after I autoclave,the lignin gets precipitated. Please suggest how to retain the solubility of lignin in the...

08 December 2020 934 3 View

What could be the reason for the discrepancies in the intracellular glucose and glycerol content in yeast grown in culture media?

I am working on bioethanol production from terrestrial and salt tolerance yeast strain.While estimating the intracellular glucose and glycerol content I have used yeast strains grown in YPD media...

29 November 2019 4,815 1 View

I was performing Filter Paper Activity using cellulase (cellic ctec 2) where I found the FPU/ ml to be 92.5. How to convert the activity into FPU/gm?

I estimated the protein concentration using Lowry method, to be 321 mg/ml. I need to add varying FPU/gm of these celullase for the enzymatic hydrolysis of lignocellulosic substrates.Please...

11 September 2019 4,507 4 View