I am trying to run QPCR on NK cell RNA using SYBR green. I have not been getting any amplification even in the 18S gene which we use as control. While the same primers and SYBR green works well on pancreatic cancer cell lines. Then I ran QPCR from the same cDNA from NK cells using TaqMan probes and got amplification and expected Ct values. What could be causing this variation?

Thanks.

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