Gurcharan Kaur

3 Questions 3 Answers 0 Followers

Questions related from Gurcharan Kaur

What could be the reason for no amplification in SYBR green QPCR while getting amplification in TaqMan QPCR?

I am trying to run QPCR on NK cell RNA using SYBR green. I have not been getting any amplification even in the 18S gene which we use as control. While the same primers and SYBR green works well on...

09 September 2018 4,750 3 View

Can anyone tell a good method to isolate RNA from NK-92MI cells?

I have been experiencing difficulties in isolating mRNA from NK-92MI cells. I am using Direct-zol RNA isolation kit right now to isolate RNA form 5 million cells per sample. After making cDNA,...

07 July 2018 281 10 View

How to do live dead imaging on tissue slices?

I have been culturing tissue slices obtained form patient tumors (I embedded tumor in agarose and sliced them using vibratome) that I need to do live dead staining on. The slices are 200 to 300...

07 July 2018 8,567 0 View