Hi, my concern is the following:
I am using the RNeasy Micro kit from Qiagen to isolate RNA from very small samples (10 embryos). Since the RNA carrier that comes with the kit is a PolyA (PA) that might interfere with downstream applications (RNA-seq in this case), we found Linear Acrylamide (LA) to be a good substitute.
We added LA to the Lysis Buffer at a concentration of 20 ug/mL (directly to the lysate in the very first step because this protocol does not give you an aqueous phase at any point, so we just followed the protocol as if we were using the PA.
Our results are confusing (see documents attached), we tried two samples (one with LA carrier (C) and one without LA carrier (NC). The traces in the Bioanalyzer seem to be better in the C group than NC but the Nanodrop shows lower concentration in C compared to NC.
Should we trust the Bio and proceed? Should we add the LA in another step of the protocol? What do you think?
Thank you so much!
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