My VCaP cell has been cultured in DMEM medium (in petri dish) for over 20 days, the cell still grows very slowly. So the medium is exchanged every 2 days. The passage of the VCaP cells has not been administered. But I checked the previous paper, the doubling time of VCaP cell line is 5-6 days. The suggestive passage condition for VCaP cells is when the confluence reaches 80-100%. Thus, I am still waiting.
Divaker Choubey
If the doubling time of cells is 5-6-days and cell confluency is 50% or lower, the medium could be changed after 3-days. I would split cells when 100% confluent as opposed to 80% with a split ratio of 1:4.
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Stefan Nikolai Andersen
I am having the same problem, any solutions would be appreciated?
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