Abel Tan

4 Questions 17 Answers 0 Followers

Questions related from Abel Tan

How to recover diluted genomic DNA?

Hi, I acidentally diluted my genomic DNA into too much TRIS HCl buffer (10x too much). I want to recover the concentrated form of genomic DNA. Is there any kit or column where I can do this?

12 December 2017 3,209 5 View

How far does $20,000 go in Outsourcing Research ?

Hi, I have a really burning project idea I want to pursue. The problem is my PI wont let me. Hence I am thinking of financing the project out of my own pocket. In order for me not to ``waste``...

07 July 2017 9,436 3 View

How do I select for integrated non dividing cells?

Hi, I am intending to do a CRISPR experiment where I knock in a GFP into a non-coding region of DNA into a non-dividing cell line using HDR. The problem is, when i transduce my cells, both...

06 June 2017 2,768 2 View

Does Reverse HDR Gene Correction Work?

Hi, Many people uses CRISPR Homology repair (HDR) to introduce a gene correction into the genome of cells using donor plasmid. They make a cut in the genome and the donor will correct the genome...

06 June 2017 483 1 View