I need to evaluate the genotoxicity of Rapamycin and I don't know which concentration should I use as some articles indicate that they use nM concentrations and others used microM.
Does 200 nM sound good for SCEs and CAs experiments?
My suggestion is to do a quick concentration based optimization.
I have worked with rapamycin in whole worms. My optimizations showed that in whole worm planaria it works at a 100nM (5 injections of 20nM rapamycin) concentration, while for C. elegans we use 100uM concentrations.
Most cell culture work uses a 20nM concentration. If your pourpose is to look for toxic effects, you could use up concentrations from this point (ex: 20nM, 50nM, 100nM, etc). This paper is good.
Rapamycin Treatment Improves Neuron Viability in an In Vitro Model of Stroke
Lauren Fletcher, Teresa M. Evans, Lora Talley Watts, David F. Jimenez, Murat Digicaylioglu
Dear Haya,
The attached chapter discusses the genotoxicity of a wide range of drugs among them rapamycin. The experiment with rapamycin was conducted in different concentrations and it was found that the effect is dose dependent. Details on the concentrations range with illustration by figures are shown.
Hoping this will be helpful,
Rafik
Rapamycin is highly selective and potent. In human primary cells and co-cultures, rapamycin has the same phenotypic impact (changes in biomarkers) from 0.5 nM to 1 microM. 200 nM should be fine (PMID:24088371).
Our studies used 100 nM in various cell types with no toxicity. Please see Liu et al. J. CIin. Invest. 2016 vol 126: p 1704.
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