What concentration of papain should I use for neuron isolation from 1 day old mice? I have papain from sigma. Also I should dilute it using what?

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How can we confirm biomolecule encapsualtion into liposome from UV-Vis or PL spectra?

I have been working with phospholipid vesicles and encapsulation of bio-molecules or drugs. But, i am unable to ensure whether it is taking place or not. what are the methods to do so including...

20 August 2020 7,076 7 View

Can microbiology pipettes be used for cell culture?

Hello everyone, We have a multichannel pipette in the microbiology lab that has been used against various pathogenic bacteria. If we want to use it for cell culture, can it be sterilised? If yes,...

08 August 2018 9,873 14 View

Why do electrons or other plasma species follow Maxwell Boltzmann distribution in plasma?

Is there any physical reason behind it or it simply because most plasmas follow classical physics.

26 January 2018 4,598 7 View

How can I connect two volumes with different material components in ANSYS APDL?

I have created a sector model for a gas turbine bladed disc. Where, the 'blade' and the 'disc' are having different material properties. 'Boolean' operation is also not possible due to complexity...

25 June 2016 6,399 2 View

How does the size, shape, and concentration of nanoparticles depend on the concentration of reducing agent?

When nanoparticles are synthesized by chemical reduction, how does the size, shape, and concentration of the nanoparticles change with the change in concentration of reducing agent?

16 May 2014 10,008 5 View

How XRD peaks change with different orientation of planes in nanopoarticles?

What is the relation between XRD peaks and orientation of planes?

03 May 2014 5,389 12 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Buffer to disrupt platelet alpha granules for PF4 quantification?

Hello! I want to quantify by ELISA the secreted (from platelets poor plasma) and the non-secreted (from platelet lysate) PF4 before and after TRAP stimulation. I will use the ELISA from R&D...

03 March 2021 1,499 2 View

Buffer Exchange using column packed with sephadex G-25 effect on protein concentration?

Hello, If i am doing a buffer exchange for an antibody of 1mg/mL, does the elution lose protein in the process of buffer exchange? For example, if i flow through 500 uL of 1mg/mL sample, and...

03 March 2021 6,299 3 View

How to get onecolumn document from standard IEEE ACCESS LateX template?

I have prepared a manuscript in IEEE Latex template, but for some purpose, I need to prepare a word file as well (detailed format is not required). Compared to two column .tex file, one column...

02 March 2021 1,830 3 View

How optimize the Size Exclusion Chromatography?

Hello! I'll do a size exclusion chromatography, but I only have an open column, and I'll perform the peptide extraction from yeast, using buffer lysis (sodium phosphate 50 mM/NaCl 30 mM/DNAse and...

01 March 2021 2,215 2 View

Why are my bands weird in this Agarose gel?

Can someone please give me some possible things that could go wrong? Here is my recipe: 0.5g Agarose 50 mL of TAE 1x 1 uL ethyl bromide. Gel was run at 100V for 1 hour. The buffer used is also TAE.

01 March 2021 9,952 3 View

IHC on mouse liver hepatocytes?

Can anyone help me choose primary antibodies for mouse liver hepatocytes and also in conjunction Cd8 cells. Also Cd3 cells. I want to look at hepatocytes and adjacent immune cells in mice with...

01 March 2021 6,953 1 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

What do I do with DLS peaks that are from the buffer?

I'm looking at the aggregation of my protein sample using DLS. Unfortunately, my buffer (20mM HEPES) also results in a set of peaks. These are at approximately 1 and 1000 d.nm. The lowest peak...

01 March 2021 9,015 2 View

How to avoid ulcers in s.c. injected tumors ?

Recently, we have injected s.c. B16F10 tumor cells in BL/6 mice. At around 12-15th day some animals showed ulcerations in tumors , with a big hole in the middle (it looks like a volcano). We have...

01 March 2021 9,938 3 View