13 Questions 17 Answers 0 Followers
Questions related from Soundarya Priya
Can someone please list the analysis that needs to be performed to understand and compare the mechanism of a protein in its native and inhibitor-bound state? (NOTE: Not the interaction between...
25 December 2022 1,283 3 View
How can I add atomic parameters for the ions Europium (Eu3+) and Americium (Am3+)
05 October 2020 1,994 6 View
I need to dock a protein with metal ions like americium and europium. How can I do this with Schrodinger
08 September 2020 7,341 2 View
I am looking fora command that will modify 3 chains available in the original pdb into a single chain and then renumber all of the residues. I have tried using alter command but when I export the...
27 June 2020 9,154 1 View
The assay is enzymatic coupled to the spectrophotometric changes in NADH absorption at 340 nm
11 November 2019 7,909 3 View
Protein ligand experiments I've been trying doesn't show a sigmoidal peak. The heat of injection is the same throughout the experiment
21 May 2019 6,179 6 View
The acid base titration standards are working fine. I just want to know if there are any protein-ligand standards. Thanks in advance
21 May 2019 9,699 3 View
I use PCR cleanup kit from Thermoscientific. The extraction of restricted plasmid from gel failed twice but the extraction of insert which was run parallely gave me DNA with good concentration and...
27 February 2019 8,151 17 View
The HiPrep 16/60 Sephacryl S-100 column that we use is clogged with some dark coloured particles at the very top. I tried cleaning up the column with 0.5M NaOH followed by distilled water. This...
28 January 2019 7,578 8 View
What is the acceptable range when checking the primers using Primer-BLAST
03 January 2019 6,558 5 View
The crucial points that need to be considered to avoid failures in cloning.
03 January 2019 6,738 2 View
Should I add oligos based on the chart flanking both the ends of the restriction site or adding oligos to the end of DNA is enough?
03 January 2019 6,608 5 View
What happens if you perform restriction digestion with an enzyme that does not have star activity but leave them for more than two days? will the overhangs be removed or chewed off? will Ligation...
18 December 2018 1,552 4 View
