Soundarya Priya

13 Questions 18 Answers 0 Followers

Questions related from Soundarya Priya

What MD analysis are required to understand the mechanism of a protein?

Can someone please list the analysis that needs to be performed to understand and compare the mechanism of a protein in its native and inhibitor-bound state? (NOTE: Not the interaction between...

25 December 2022 1,350 3 View

How to add atomic parameters in Autodock?

How can I add atomic parameters for the ions Europium (Eu3+) and Americium (Am3+)

05 October 2020 2,199 6 View

How do I dock a metal ion to a protein in Schrodinger?

I need to dock a protein with metal ions like americium and europium. How can I do this with Schrodinger

08 September 2020 7,396 2 View

How to modify and merge multiple chain into a single chain in Pymol?

I am looking fora command that will modify 3 chains available in the original pdb into a single chain and then renumber all of the residues. I have tried using alter command but when I export the...

27 June 2020 9,259 1 View

What commercially available enzyme can be used as a positive control for ATPase assay?

The assay is enzymatic coupled to the spectrophotometric changes in NADH absorption at 340 nm

11 November 2019 7,965 3 View

How to choose the concentration of protein and ligand for ITC experiment?

Protein ligand experiments I've been trying doesn't show a sigmoidal peak. The heat of injection is the same throughout the experiment

21 May 2019 6,229 6 View

Are there any standard protein-ligand titration reactions that I can use to calibrate my ITC equipment?

The acid base titration standards are working fine. I just want to know if there are any protein-ligand standards. Thanks in advance

21 May 2019 9,733 3 View

Can PCR cleanup kits be used for gel elution of restricted plasmid DNA?

I use PCR cleanup kit from Thermoscientific. The extraction of restricted plasmid from gel failed twice but the extraction of insert which was run parallely gave me DNA with good concentration and...

27 February 2019 8,226 17 View

How to unclog FPLC column?

The HiPrep 16/60 Sephacryl S-100 column that we use is clogged with some dark coloured particles at the very top. I tried cleaning up the column with 0.5M NaOH followed by distilled water. This...

28 January 2019 7,623 8 View

What are the desired Self complementarity and Self 3' complementarity values?

What is the acceptable range when checking the primers using Primer-BLAST

03 January 2019 6,615 5 View

What are the points I need to take care when designing primers to amplify GOI which will be used for cloning?

The crucial points that need to be considered to avoid failures in cloning.

03 January 2019 6,772 2 View

Is it necessary to add oligos front and back of the restriction sites?

Should I add oligos based on the chart flanking both the ends of the restriction site or adding oligos to the end of DNA is enough?

03 January 2019 6,652 5 View

What happens if you do restriction digestion for longer time?

What happens if you perform restriction digestion with an enzyme that does not have star activity but leave them for more than two days? will the overhangs be removed or chewed off? will Ligation...

18 December 2018 1,595 4 View