One of the most novel molecular techniques is CRISPR. I have heard that it is being widely spread among molecular biologists and can significantly shorten time needed to conduct research (eg. building animal models). Additionally, the use of CRISPR has recently been able to open an interesting debate in top ranking journals about editing even human germline. Mycorrhizal ecology has wisdom a revolution due to advances in molecular techniques. That is why such a technique called my attention and I wonder what the likely potential of CRISPR can be for my current research area (mycorrhizal ecology) in the next future.
you know Myorizal fungi and any modification or transgenic or cloning need more cycle in these not direct used in fungi need more steps I think that
Dear Raghad Mouhamad,
First of all, I would like to thank you very much for your reply. I agree that mycorrhizal fungi are complex organisms. However, Crispr seems promising. It may help mycorrhizal ecology to solve many questions. Therefore, may I ask your opinion about the steps that should be followed to optimise such a tecnique for the use with mycorrhizal fungi?
Hi David,
Please see this recently published paper (2015) below. The paper describes how to use Genome editing tools for the applications of evolutionary and ecological studies. Although it did not mention mycorrhiza asked in your question, but you can get a sense of the direction of how to use these tools for ecological studies.
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