Why my RNA is not good?

I am working in a laboratory with Cov19. Extraction is doing kit but it isn't good and the amount RNA, 260/280 is higher than 3. Can someone help us in this regard?

12 September 2020 2,827 7 View

Hello . Is there any stereo video dataset to produce depth ?

I'm going to produce depth video using a video with the right camera and a video with the left camera,(I mean two videos taken with the stereo camera).

16 October 2019 5,014 3 View

Proving the formation of NiCoFe2O4 nanoferrite by XRD?

Hi I synthesized NiCoFe2O4 nanoferrite. I used XRD to prove the formation of NiCoFe2O4 nanoparticles. I understood that the position of the peaks is correct. I mean that the nanoferrite powder...

07 December 2016 7,735 16 View

Hydrogen peroxide effect on vitamin c?

does vitamin C and H2O2 have any chemical reactions which will prevent us from detecting Vitamin C?

15 August 2016 1,715 3 View

I have trouble in detecting my protein of interest in total cancer cell line lysate in western blot. How should I solve this problem?

I can detect beta actin band and my cell line is MDA-MB-231 breast cancer cell line.

29 September 2015 7,619 20 View

How can I find a displacement table from stress-strain curve in order to use it in damage evolution in Abaqus?

I'm working on uniaxial tensile test for an epoxy and crack propagation in it. I have data from my lab work and now I would like to model it in Abaqus. I made the bone shape sample and everything...

03 February 2015 8,650 1 View

What is the difference between the prevalence of insulin resistance found in those with NASH versus those with Hepatitis C?

NAFLD shares many overlapping features with the insulin resistance syndrome, also known as "Syndrome X" including central or visceral obesity, type II diabetes, and dyslipidemia. Of course, NAFLD...

14 September 2014 882 5 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Does any one know how i can prepare liver tissue with transmission electron microscope with good fixation?

i have problem in preparation of liver tissue with bad cell organelles and how i can adjust osmolarity

03 March 2021 8,890 1 View

How does the gut microbiome affect the fibrotic process within the liver?

and Can the gut microbe be used for the treatment or diagnosis of liver fibrosis?

02 March 2021 557 2 View

IHC on mouse liver hepatocytes?

Can anyone help me choose primary antibodies for mouse liver hepatocytes and also in conjunction Cd8 cells. Also Cd3 cells. I want to look at hepatocytes and adjacent immune cells in mice with...

01 March 2021 6,953 1 View

Isolates of Hymenoscyphus fraxineus?

Dear Dr. Cai, my name is Simone Prospero and I work in the team of Phytopathology at the Swiss Federal Institute for Forest Snow and Landscape Research (WSL;...

01 March 2021 1,133 1 View

Is it harmful to breed for Cre Homozygosity initially?

Hello, I am currently using a Cas9 knock in system that will be expressed in the liver. To do so STOP codon fl/fl Cas9 KI mice were bred with Albumin-Cre mice to generate heterozgous pups, that...

24 February 2021 5,759 3 View

How should I go about evaluating cytoplasmic and nuclear Arginase-1 activity?

Hello! I've stained some bright field Arginase-1 Hematoxylin slides, but I'm having a hard time finding any computed histology predicates for evaluating the intensity of the cytoplasm verses the...

03 February 2021 3,039 1 View

Does euthanasia by CO2 inhalation cause NF-kB activation in mouse heart or body in general?

I was wondering if euthanizing mice by carbon dioxide inhalation would cause activation of NF-kB inflammatory response?

26 January 2021 4,096 2 View

How many ug should I choose as the protein amount when doing western blot in liver tissue?

25 January 2021 794 3 View

Can fibrosing cholestatic hepatitis in an immunocompromised patient be diagnosed without a liver biopsy?

provided that all the criteria for diagnosis has been fulfilled and alternative diagnosis are also ruled out. The patient has coagulopathy and transjugular biopsy wont be possible due to resource...

13 January 2021 8,935 4 View