Hi, I am going to study the DNA1(21mer) bonding to DNA2(21mer+aptamer conjugated to it(42mer)) through EIS. DNA1 is immobilized on the surface of my gold working electrode through thiol group affiliation to gold.
I am applying EIS in each immobilization step. based on the literature in each step, the Rct increases. But in my case, in DNA2 to DNA1 bonding step, it decreases. Does anybody know what will be the problem?
Thanks for your reply. What do you mean exactly by reaction condition? The pH is 7. the solvent is PBS.
What kind of surface gold working electrode are you employing Zahra? It`s a commercial electrode, a hand made electrode, etc?
Zahra are you using (Fe(CN)6)-3 as electrochemical reporter?
The same has happen to me using screen printed electrodes with electrochemical gold deposition.
Yes I am using ferrocyanide solution as my probe. So, how did you fix it? Or is there any fact behind it?
Perhaps you could explore your system in other gold electrode (commercial for exemple). I`m not an explanation yet for this fact, I`m sorry Zahra.
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