16 Questions 9 Answers 0 Followers
Questions related from Chandra Kishore
I am performing Cut & Tag experiment. I was wondering if there is any alternative/replacement for TAPS pH 8.5 in single tube Tagmentation and pA-Tn5 release ( DNA purification) step of Cut and...
03 April 2024 4,183 0 View
Most of the Antibody company (having pr. Ab conc. range 0.2 mg/ml - 0.5mg/ml) websites suggests dilution around 1:200, but it seems not staining or faintly staining. What is the hand on experience...
29 March 2024 150 3 View
I am growing small cell lung cancer suspension cells. How do I separate dead cells from live cells while splitting the cells after confluency in flask?
29 January 2024 8,797 6 View
Human dermal fibroblast cells are seeded to transfect using Lipofectamine2000 for generating iPSCs. plasmid/lipofectamine ratio 2:1. 15% FBS and pen/step antibiotic 500 microlitre per 100 ml.
08 April 2021 3,386 5 View
I want to study the effect of drug on TGF beta and NFkB pathways in SW480 and SW620 cell lines.
29 December 2016 8,909 2 View
After a few passages I am getting change in morphology. When I had changed the serum it had also shown some changes in cell proliferation and growth pattern.
31 October 2015 6,419 10 View
I am observing some change in growth pattern and morphology
31 October 2015 4,655 5 View
Protocols disagree on the exact voltage and current set up for the above technique
10 December 2014 1,840 7 View
My drug is getting precipitated.
07 October 2014 7,458 5 View
I need hesperidin conc of 100,200,300,400 microM.I am doing following - Made 100 mM hesperidin in DMSO. Then took 1 micro L (100mM hesperidin) + 1000 micro L media i.e 100 microM. 2microL(100mM...
14 September 2014 5,343 5 View
Is it ok if ic50 is 1mM for the phytochemical drug ?
31 July 2014 1,042 6 View
I am able to get my ic50 only at this conc., 0.1% is not giving ic 50 at conc. of drug shown in literature.
01 July 2014 1,872 5 View
Should a drug in liquid form (Geraniol) be first dissolved in solvent (DMSO/Ethanol) then diluted in media or directly added to media to make different concentrations for an MTT assay? Geraniol...
14 June 2014 7,414 4 View
For HCT 116, the modal number at 45 is 62 %. What does it mean ??
22 January 2014 7,384 2 View
Any example where micro RNA is involved in regulation, modification or interaction with DNMT, HM, HDACs or any other epigenetic regulator?
08 November 2013 509 6 View
The process of formation is almost the same for the both of them. Why exactly are they named differently?
27 October 2013 8,596 3 View