Chandra Kishore

19 Questions 15 Answers 0 Followers

Questions related from Chandra Kishore

What can be the alternate buffer for TAPS (PH 8.5) in Post tagmentation Wash step in Cut & Tag experiment ?

I am performing Cut & Tag experiment. I was wondering if there is any alternative/replacement for TAPS pH 8.5 in single tube Tagmentation and pA-Tn5 release ( DNA purification) step of Cut and...

03 April 2024 4,255 0 View

How much of primary antibody do you add on slides in immunohistochemistry(IHC) / Immunocytochemistry (ICC)?

Most of the Antibody company (having pr. Ab conc. range 0.2 mg/ml - 0.5mg/ml) websites suggests dilution around 1:200, but it seems not staining or faintly staining. What is the hand on experience...

29 March 2024 296 3 View

How to separate dead cells from live cells when propagating suspension cells?

I am growing small cell lung cancer suspension cells. How do I separate dead cells from live cells while splitting the cells after confluency in flask?

29 January 2024 8,855 6 View

Can Human dermal fibroblast cell line used to form iPSC or only primary dermal fibroblast can be reprogrammed?

In literature, I can find only primary human fibroblast cells being used for reprogramming.

07 August 2021 3,473 10 View

Why are we getting very high toxicity while transfecting human dermal fibroblast cell line using Lipofectamine 2000?

Human dermal fibroblast cells are seeded to transfect using Lipofectamine2000 for generating iPSCs. plasmid/lipofectamine ratio 2:1. 15% FBS and pen/step antibiotic 500 microlitre per 100 ml.

08 April 2021 3,437 5 View

What concentration of TGF beta and TNF alpha should i use in SW480 and SW620 cell lines for pathway induction

I want to study the effect of drug on TGF beta and NFkB pathways in SW480 and SW620 cell lines.

29 December 2016 8,952 2 View

How many passages of colon cancer and cervical cell line can be maintained in the same dish or flask continuously without affecting the assays?

After a few passages I am getting change in morphology. When I had changed the serum it had also shown some changes in cell proliferation and growth pattern.

31 October 2015 6,477 10 View

Is there any effect of change in serum on cancer cell line?

I am observing some change in growth pattern and morphology

31 October 2015 4,697 5 View

Why should we run SDS-PAGE on constant voltage and Western Blot transfer on constant current ?

Protocols disagree on the exact voltage and current set up for the above technique

10 December 2014 1,890 7 View

Does dmso of different grades make a difference in phytochemical drug solubility? Which is the best grade to solubilize phytochemical drug?

My drug is getting precipitated.

07 October 2014 7,503 5 View

Hesperidin is getting precipitated - How can I solve this problem?

I need hesperidin conc of 100,200,300,400 microM.I am doing following - Made 100 mM hesperidin in DMSO. Then took 1 micro L (100mM hesperidin) + 1000 micro L media i.e 100 microM. 2microL(100mM...

14 September 2014 5,387 5 View

What maximum IC-50 conc. of phytochemical drug in MTT is acceptable for publication?

Is it ok if ic50 is 1mM for the phytochemical drug ?

31 July 2014 1,085 6 View

Can anyone tell me is it OK to use 0.6 % highest conc. of DMSO in MTT for SW 620 and SW 480 cell lines?

I am able to get my ic50 only at this conc., 0.1% is not giving ic 50 at conc. of drug shown in literature.

01 July 2014 1,916 5 View

Geraniol directly dissolved in media not giving effect in MTT- Can anyone help?

Should a drug in liquid form (Geraniol) be first dissolved in solvent (DMSO/Ethanol) then diluted in media or directly added to media to make different concentrations for an MTT assay? Geraniol...

14 June 2014 7,459 4 View

What does modal number of a cell line mean?

For HCT 116, the modal number at 45 is 62 %. What does it mean ??

22 January 2014 7,429 2 View

Why does BCR-ABL mRNA in blood show decline when treated with Gleevec in CML patients?

In CML patients it has been observed that BCR-ABL mRNA shows decline in response to Gleevec treatment.

15 November 2013 7,325 7 View

Does the micro RNA act as a epigenetic modifier?

Any example where micro RNA is involved in regulation, modification or interaction with DNMT, HM, HDACs or any other epigenetic regulator?

08 November 2013 553 6 View

Is there any example of down regulation of micro RNA gene transcription by its promoter CpG hypermethylation in cervical cancer cell lines?

Epigenetic down regulation of micro RNA gene has been discovered in many types of cancers.

08 November 2013 1,893 1 View

What is the difference between siRNA and micro RNA?

The process of formation is almost the same for the both of them. Why exactly are they named differently?

27 October 2013 8,640 3 View