I am performing a metagenomics based experiment, where I need to send the sample to an external facility for NGS. The transit is of approximately 7 days. What can be the extraction and transfer protocol that can be followed?
Extract the DNA with any standard DNA isolation from soil kits and transport on dry ice.
Hi,
To get a productive amount of DNA from your environmental samples, you can use Qiagen DNeasy Powersoil Kit to extract the metagenomes. I did use this kit and got a brilliant result after sending it to my external NGS facility.
If your concerned samples are soil or sediment or any other naturally-governed origin, then you must use specific kit to remove pollutants or strong organic matters hindering the DNA isolation process. Using any simple DNA isolation kit might not gives you all the extractable DNA confined to those natural media.
For transportation, you can certainly use dry-ice in a tightly sealed container and that will roughly give you a 3-4 days of time (as in my case, it was 4 days and samples were fine in it).
Best,
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