What buffer should I use for insulin at pH 5.3?

04 June 2015 1 3K Report

I am trying to make insulin solution in sodium citrate buffer at pH 5.3. I notice that the solution becomes cloudy. Is this due to zinc precipitation? Will sodium acetate be a better buffer?

Tausif Alam

You can use dilute HCl (0.001-0.01N) to dissolve insulin, aliquot in portions suitable for your work and store them frozen. Insulin is quite stable under these conditions at high concentrations (1mg/ml or higher).

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