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I have imaged aggregated protein fibrils using AFM as a part of a project that is under review. The reviewer has asked to provide information on fibril strength and mass per unit length. I have no...
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I have generated electrostatic potential maps of a protein at different pH values using a software. I am an experimentalist and hence I am not quite sure how to interpret the blue-white-red colors...
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I am trying to make insulin solution in sodium citrate buffer at pH 5.3. I notice that the solution becomes cloudy. Is this due to zinc precipitation? Will sodium acetate be a better buffer?
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I am facing primarily 2 issues with Western Blot. Firstly, during the transfer process, lot of heat is getting generated that results in boiling I do put ice pack during the process, but lately...
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