Hi, does anyone have experience with proteomic analyzes using the PEPMAP NEO C18
2UM 75UMX500MM 1500 BAR combined with Dionex, Ultimate 3000? What are your pressures at 4% ACN at a flow rate of 300ul and 400 ul, please? Thanks a lot.
Dear all, I would like to ask if there is anyone who can analyze the MOF framework based on a cif file. I need to analyze four MOF compounds using Schlafli symbol (the point symbol of the crystal...
21 July 2020 9,991 5 View
My main research interest is focused on sustainable multifunctional component of the city urbanized area. Method of the doctoral thesis is based on research by design applied on main university...
11 September 2018 5,530 8 View
How can I use Mascot for the interpretation of results as fold-change protein in quantification? it is possible to determine through spectral counting values obtained from the matches?
18 August 2018 6,281 6 View
Hello, I recently tried to get the mRNA sequence of WIP1/PPM1D gene from NCBI, but it does not contain uracils. Therefore I thought the listed mRNA sequence was actually a cDNA. So I used BLAST to...
30 June 2018 519 1 View
The genome of S. cerevisiae was the first completely sequenced from a eukaryote. It was released in 1996. Life with 6000 Genes What kind of obstacles poses mitochondrial DNA for sequencing?
05 June 2018 9,657 3 View
We have scalar and cross product. Cross product works in 3D only. But why not define a torque in 2D? Or 4D? Imagine now that we don't know anything about products of vectors. How to multiply? For...
24 July 2016 8,966 27 View
Found in Neogene deposits.
07 July 2016 7,100 9 View
19 March 2015 736 2 View
To what extent can we decrease the dimensions of particles so that solid-solid phase transition can be detected? Not only by XRD, but also by DSC, IR, NMR... In other words what is the smallest...
01 January 1970 5,731 3 View
Hallo, please can i ask you... is it possible to use only sonication instead of RIPA buffer to prepare the sample (isolation of heart mitochondria) for LC/MS analysis? When using RIPA is it...
01 January 1970 6,414 6 View
When I conducted the degassing experiment using decompression under anaerobic condition(and I used ferrous based chelating agent), the oxidized ferric were observerd. So, could you tell me the...
03 March 2021 4,892 5 View
Hello, If i am doing a buffer exchange for an antibody of 1mg/mL, does the elution lose protein in the process of buffer exchange? For example, if i flow through 500 uL of 1mg/mL sample, and...
03 March 2021 6,299 3 View
We use reactive sputtering process to make aluminum nitride film, and want to make c-axis aluminum nitride film on a silicon substrate, but the crystal orientation cannot be seen with...
03 March 2021 7,975 3 View
03 March 2021 5,360 2 View
I am using a 2707 waters HPLC device. When I try to inject a sample, it says missing plate or rack. I changed the needle and calibrated its position but I still get the same problem. I even get...
02 March 2021 1,408 1 View
We found tests to determine cardiovascular risk taking into account lipid profile, blood pressure, age, history of diabetes, other laboratories. But when weight is involved, there is a paradox. I...
02 March 2021 6,036 1 View
Hello, I have a mechanism where air rises due to convective flows. In this simulation, nothing is driving the air except this. I understand that the Boussinesq approximation only works for small...
02 March 2021 4,910 3 View
(This statement is from wikipedia, BTW). What is so special about metallic bond that is limiting high-P low-T state of a matter (as long as individual atoms exist, not white dwarf of neutron star...
02 March 2021 3,309 2 View
I took measurements of different concentrations of 15 nm and 5 nm Au nano particle solutions. I'm trying to determine which concentration I measured gives the best reading overall. I also...
02 March 2021 7,626 1 View
I am using a Sutter electrode holder for my patch clamp experiments, but I'm having trouble keeping positive pressure inside my electrode due to a leak out of the back of the holder. I have added...
02 March 2021 5,829 1 View