Hi, does anyone have experience with proteomic analyzes using the PEPMAP NEO C18
2UM 75UMX500MM 1500 BAR combined with Dionex, Ultimate 3000? What are your pressures at 4% ACN at a flow rate of 300ul and 400 ul, please? Thanks a lot.
I would like to ask what type of input data do these commands require. I am a little bit lost in the Phylonet manual. Is it possible to construct phylogenetic network in Phylonet using data such...
14 November 2023 2,434 0 View
Specifically, what is the difference between A) POPDATA=1, LOCPRIOR=1, LOCDATA=0, LOCISPOP=1 and B) POPDATA=0, LOCPRIOR=1, LOCDATA=1, LOCISPOP=0 ? For the clustering algorithm and calculating...
27 September 2023 5,275 2 View
Why Heat capacity and Entropy are different quantities when they have both the same units? It means they are the same.
25 April 2023 4,846 6 View
Was there observed some new smart solution caused by the coincidence in nature now?
31 March 2023 999 1 View
Please, can anybody send a link (if it exists) where I can learn what is the difference between FactSage, ThermoCalc or Pandat (or others) when calculating phase diagrams? Or to summarize the...
11 August 2022 7,507 4 View
Dear all, I would like to ask if there is anyone who can analyze the MOF framework based on a cif file. I need to analyze four MOF compounds using Schlafli symbol (the point symbol of the crystal...
21 July 2020 10,200 5 View
I am interested to know more about BIM (Building Information Modeling).
02 February 2019 8,782 13 View
I work on research on evaluation of active labor market policies. However, I have found few studies to deal with the qualitative evaluation of these policies by involving the participants...
08 December 2018 8,959 1 View
My main research interest is focused on sustainable multifunctional component of the city urbanized area. Method of the doctoral thesis is based on research by design applied on main university...
11 September 2018 5,658 8 View
How can I use Mascot for the interpretation of results as fold-change protein in quantification? it is possible to determine through spectral counting values obtained from the matches?
18 August 2018 6,420 6 View
A crude extract of fungal culture using EtOH was subjected to column and TLC and partially purified compound was obtained. UV vis spectrum of the compound/s has max absorbance at 218nm. The...
11 August 2024 9,801 2 View
Can anyone explain this method? Especially the last statement where it says only at 1.5 to 2.5mins was the MS/MS connected to the UPLC. How is that possible, is it a feature in this specific...
11 August 2024 8,141 3 View
Hello experts, Does anyone know any free software about retention index prediction ?
08 August 2024 7,403 2 View
I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...
08 August 2024 9,882 2 View
I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...
07 August 2024 505 3 View
I have been getting a low plasma and no coating while doing rf sputtering of copper doped ZnS using a power of 140 watts and at pressure of 6.5x10-3 Mb.
07 August 2024 4,526 4 View
I am trying to recover liquid CO2 from a mixture of 0.6 CO2, 0.3 N2, and 0.1 O2. My aim is to recover about 99% liquid CO2 at the bottom of the column and make sure the amount of gaseous CO2...
06 August 2024 4,611 0 View
Hello, I suspect that a molecule I am using is causing bacteria to experience osmotic shock. What chemical properties should I look for to compare this capability with those of other substances?...
06 August 2024 977 1 View
I am analyzing cherts from different sources found within one specific geologic formation using ICP-MS/ES. My committee was asking this question about my analysis. I was not sure how to answer this
05 August 2024 7,902 3 View
Have you ever seen a LC-MS/MS method uses both internal standards and external standards (in matrix matching purpose) but the concentrations of internal standards are outside the calibration curve...
05 August 2024 3,084 6 View