Miroslav Ferko

4 Questions 6 Answers 0 Followers

Questions related from Miroslav Ferko

Submitting proteomics data to ProteomeXchange via PRIDE?

Has anyone had experience submitting proteomics data to ProteomeXchange via PRIDE? I’m working with mass spectrometry data from multiple sample groups. For each group, I have: .baf and .mgf...

last month 8,511 2 View

What are your pressures at 4% ACN at a flow rate of 300ul and 400 ul, on Pepmap neo 1500bar, 50cm?

Hi, does anyone have experience with proteomic analyzes using the PEPMAP NEO C18 2UM 75UMX500MM 1500 BAR combined with Dionex, Ultimate 3000? What are your pressures at 4% ACN at a flow rate of...

07 July 2023 9,775 0 View

Mascot use for quantification via fold change ?

How can I use Mascot for the interpretation of results as fold-change protein in quantification? it is possible to determine through spectral counting values obtained from the matches?

19 August 2018 6,447 6 View

RIPPA Buffer, sonification, SPE columns

Hallo, please can i ask you... is it possible to use only sonication instead of RIPA buffer to prepare the sample (isolation of heart mitochondria) for LC/MS analysis? When using RIPA is it...

01 January 1970 6,557 6 View