Hello everyone,
I have a small query on ChIP that I have extracted chromatin and performed sonication to obtain 200-500 bp fragments successfully from rice leaf tissue. However, I have observed an intense band with the size of ~800bp in both sheared and non-sheared (control) samples (file attached below). Can it be RNA or DNA or both? (or) Is this indication of successful or unsuccessful cross-linking? Did anyone familiar with it?
Please do the needful, and thank you for taking the time to read this. I look forward to hearing from you shortly.
Hi. Smear above the DNA band indicates the contamination of the RNA meanwhile smear below the DNA band indicates the contamination of the protein.
You can get the answer from here.
https://geneticeducation.co.in/a-complete-guide-for-analysing-and-interpreting-gel-electrophoresis-results/
Siti NABILAH Mohamad Shudirman Hi Siti, thanks for answering my query. I think I have to do RNAse and Protease treatment for these samples before running them on agarose gel.
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