Kunchapu Chennakesavulu

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Questions related from Kunchapu Chennakesavulu

What are the nanodrop reading values of DNA after de-crosslinking in ChIP?

Hi everyone, Here are the nanodrop reading values for DNA concentration after the chromatin de-crosslinking step followed by elution with 20 uL elution buffer. Sample ng/uL ...

23 June 2022 6,114 0 View

What is the % input control in ChIP?

I am confused with the % of input control in ChIP. How much amount of sheared chromatin has to be taken as a control for ChIP-qPCR? Let's say for example, I have 350 uL sheared chromatin samples...

18 June 2022 5,551 0 View

Can we consider Bi-allelic mutation as a homozygous mutation?

Since both alleles of the same gene are getting edited in Bi-allelic mutants of any particular organism, Can we consider that organism as a homozygous mutant for the gene?

18 May 2022 2,592 3 View

What is the probability of getting transgene-free genome edited plants in T1 and T2 generations?

Since the T-DNA insertion copy number decides the chances of getting transgene-free genome edited plants at T1 generation, how can we identify the copy number of T-DNA insertions at T0? And what...

18 May 2022 7,338 2 View

what are those non-specific intense bands in sheared or non-sheared (control) rice leaf chromatin samples in agarose gel electrophoresis?

Hello everyone, I have a small query on ChIP that I have extracted chromatin and performed sonication to obtain 200-500 bp fragments successfully from rice leaf tissue. However, I have observed an...

12 April 2022 8,933 2 View