The image attached is a streak plate for DH5a cells on a non-antibiotic plate. I have white colonies visible at the start of my streaking [ I drew an initial deposit]. I also see these white colonies in the transformed plate with carbenicillin [image not attached]. In addition, when non-antibiotic fresh plates were incubated without inoculation, 3-4 colonies were found. Do my incubator needs cleaning?
IMHO, there might be some minor contamination, also the whiter, separate colonies on the plater do not seem to be the same strain/specie of bacteria.
Knowing this much about situation I would recomend filtering of air in the room and more aseptic techique. Still may get effective results of Your work Good luck!
You may look into the article here. Hope it helps
https://doi.org/10.1007/s13762-024-05635-3
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