Suba SOUNDARYA Santhi Anandhan

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Questions related from Suba SOUNDARYA Santhi Anandhan

How to stain lipid droplets using bodipy?

I opted bodipy due to its smaller stoke’s shift (493/503) but my images do not have consistent results. in the same well few fields are clean without background and other fields have background....

12 June 2024 3,516 3 View

How to do a good immunofluorescence on primary neuronal culture? How to prepare the IC solution for immunofluorescence?

One realisation is that my protocol with 10%NGS in PBST is not working as well as the IC solution. I need to prepare that solution independently but I do not have the recipe. My protocol is...

04 August 2023 1,603 0 View

How to dissolve the precipitated DNA after EtOH purification? Should I increase the washes in the purification step?

I amplified the fragment from the plasmid and do EtOH precipitation. After I air-dry them for an hour I reconstitute them in the buffer. The pellet is not dissolving even at 55C for an hour. The...

19 December 2022 2,438 2 View

What are these white colonies on the start of my streak plate [refer image] and transformed plate [image not attached]? Do incubator needs cleaning?

The image attached is a streak plate for DH5a cells on a non-antibiotic plate. I have white colonies visible at the start of my streaking [ I drew an initial deposit]. I also see these white...

22 July 2022 3,727 2 View