What's the role of bioinformatics in poultry science?

bioinformatics has a big importance in human science and lots are seeking to study that field, so it attracts me to know it's relation with poultry science and how we can benefit from it in...

20 January 2021 6,963 3 View

2000 DARPA dataset ?

Hello everyone, I would like to use 2000 DARPA dataset (http://www.ll.mit.edu/r-d/datasets/2000-darpa-intrusion-detection-scenario-specific-datasets) to evaluate my method to detect multi-step...

08 December 2020 7,922 2 View

What is wrong with the uploaded file (Scf input file Quantum ESPRESSO software)?

Hello dear friends I have little experience working with Quantum Espresso software. I created an input file for this software for calcium titanate. But this input gives an error. I do not know...

29 August 2020 3,729 3 View

Removing noise using Scipy.signal.butter?

I am going to remove the noise from a brain recorded signal. It was a continuous recording and with sample rate=30kHz, it was digitized. So now it is a digital signal. I have written the code here...

18 February 2020 7,857 2 View

Etchant for 5754 alloys?

What is the best etch solution for aluminum 5754?

26 December 2019 714 3 View

How do peptide vaccines not be destroyed by proteases after injection into mice?

How do peptide vaccines not be destroyed by proteases after injection into mice? What assurance is there that the peptide vaccine will not be eliminat

12 December 2019 4,370 3 View

Cutting Si3N4 microparticle?

I have microparticles of Si3N4 that is coated with cupper using electrolees method. What does method can be use to determine the probably phases from center of powder to external surface and...

07 December 2019 4,846 5 View

Is the value 0.03 supposed medium effect size or small effect size for the sample size=17 on the basis of Cohen1988 PP.284-7?

How do you interpret the values between 0.01 and 0.06 for a sample size = 17? small or medium? in my sample size, there is no significant difference between the mean sores two groups. the values...

28 June 2019 5,273 3 View

What is subtantiveness in content?

I think, it means completeness, i.e, writer mention majour events, setting, character' acts or behaviours, and out come of story. Am I right?

24 April 2019 7,587 3 View

What is a reliable, and validate grammar recognition test?

For my research goals, I design a grammar test in such way that it tests some grammar knowledges in the special areas, though frequently reflects story context which is used for teaching...

24 February 2019 10,007 2 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

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02 March 2021 3,060 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

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28 February 2021 9,936 2 View

Has anyone had luck using cell lines other than HEK 293 (such as COS1) to package lentivirus?

I am looking to package lentivirus in endothelial cells but I am opposed to using 293 cells due to personal ethics regarding the source. I have done some reading and saw that COS1, COS7, & CHO...

25 February 2021 3,030 1 View