Is it possible that a protein band appears on a western blot but not detected on ELISA?

I am working on recombinant protein expression in E. coli. Recently, I thought I had expressed a certain protein successfully as I could see a band of the expected size - 17 KDa (this band was...

14 October 2020 5,144 4 View

Is it possible to fix a co-culture of bacteria and human cells in 96 well plates and use the plates at a later point for flow cytometry analysis?

I am co-culturing bacterial strains with human cells (WBCs) and there are about 3000 different bacterial strains. The idea is to carry out flow cytometry experiments with these samples (in 96 well...

09 July 2020 3,106 5 View

How to isolate diazonium tetrafluoroborate ?

I'm trying to synthesis and isolate diazonium salt of tetrafluoroborate. this salt showing white color as per reported paper. while I'm doing after addition of NaNO2 colour showing faint yellow...

11 February 2020 4,590 2 View

How to calculate Eigen sensitivity matrix for structural dynamic problems?

I am solving finite element model updating problem. In order to achieve that i am studying inverse Eigen sensitivity method as a updating method. There, i am unable to understand how to calculate...

04 February 2020 4,513 4 View

What are the techniques to calculate impurity concentration in a metal?

Hello, Can anyone inform me on the techniques (other than semi grand canonical or grand canonical ensembles) that one can use to calculate the equilibrium impurity (say P) concentration in...

21 November 2019 5,137 3 View

Information extraction from unstructured documents?

I am looking for algorithm(s) to extract data and its value from unstructured documents. We were given list of tokens against which the information from the documents to be extracted. Going...

19 October 2017 9,065 3 View

How to calculate the amount of different phases in a composite?

Hi all, I have composites of magnesium silicate and magnesium titanate. I have confirmed from the XRD data that, they have different phases with the change in the composition. I want to calculate...

22 May 2016 1,566 2 View

How enhance transfection in CHO cells to express fusion proteins of approx. 90-100 KDa in size?

13 August 2015 5,668 4 View

Any recent or past work on anti tarnish coating on silver and copper?

I want to know conventional methods available to remove the tarnish as well as prevention methods of tarnish of metals.

22 December 2013 7,458 1 View

What is the role of the complexing agent in sol-gel process?

I have prepared two sols, one with complexing agent and other without complexing agent. Both the sols are stable and transperent, but when deposited films on glass substrate using dip coating, sol...

24 October 2013 7,753 9 View

Why almost every element would be metallized in sufficiently high pressure and low temperature?

(This statement is from wikipedia, BTW). What is so special about metallic bond that is limiting high-P low-T state of a matter (as long as individual atoms exist, not white dwarf of neutron star...

02 March 2021 3,309 2 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Which is a lucid standard comprehensive book to guide on psychological scale construction and its standardization?

02 March 2021 7,082 3 View

LOAD DISPLACEMENT CURVES Stiffness increasing in ANSYS non linear analysis. HOW CAN I CORRECT IT?

Hi. I am doing non linear FE analysis using a new material beam under flexural 2 point loading using ANSYS. I have defined a bilinear material model. The solution has converged. In results I am...

01 March 2021 9,911 3 View

How to choose ANN number of hidden layers and nodes?

Hello, Could you please share any interesting research explaining how to choose the number of hidden layers and nodes per layer in case of regression problems using ANN? Thank you, any help would...

01 March 2021 6,200 3 View

PUC19 as a transfer plasmid for lentiviral delivery?

Hello, Is it possible to use pUC19 as a transfer vector to be packed in using the second generation viral particles packaging system( pMD2.G; psPAX2 plasmids)? As far as I understand it there is...

28 February 2021 4,868 2 View

How could I take into consideration the weights of observation in a system of seemingly unrelated regressions (SUR) in rstudio?

I build a model of 4 regression equations (probit and OLS) in R studio. I need to take the weights of observations into consideration. While it is simple to do so for every regression equation,...

27 February 2021 1,233 1 View

Can random sampling have inclusion criteria?

As random sampling means that everyone in the population have an equal chance to be chosen, can it have inclusion criteria? My research participants have been chosen based on the inclusion...

26 February 2021 6,905 10 View

Anyone that can help with Maxquant TMT16plex which is missing?

Hi I am very new to proteomics analysis. I have got raw files from core facility running thermofisher TMT 16plex 4 hours. I cannot find on maxquant the option TMT16Plex there is only up to 11...

25 February 2021 1,675 3 View

Can I use TCA instead of TFA for protein analyis in RP-HPLC?

I am trying to analyze lipase using the reverse phase-HPLC C18 column. In the literature, 0.1 % TFA was and 60% ACN was used. Can I use TCA in place of TFA? If any other methods are available to...

25 February 2021 2,388 3 View