I am trying to extract my membrane bound plant protein with different extraction buffers but i am unable to extract most of it. So can anyone suggest a extraction buffer ?
Hi
Extraction of membrane proteins usually involves a similar procedure, with isolation of the cell membrane followed by extraction of membrane proteins from them. I suppose you can do the following:
Following this, you will have to optimize your detergent for size exclusion chromatography separately.
you might also need to consider different tags at different positions if you feel that a certain domain that harbours the tag is getting cleaved off during the purification process, resulting in low yield.
Arunabh Athreya thank you for your detailed explanation. i will go through this and use it
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