I mixed ICG and DSPE -PEG in water (DSPE-PEG solution is in chloroform) and then measured the UV-spectrum, I expected to see the shift of about 5nm but I am getting the shift of 15nm. ICG conc is 5uM and the ratio taken is 1:16 (ICG : DSPE-PEG).
What could be the reason?
I could not find the any paper where this particular reaction is explained.