I'd like to isolate Alternaria from plant leaves. Which media is best for rapid fungal growth as well as sporulation ? Are there any pre-requisitics growth conditions in order to induce sporulation? What is the color of Alternaria culture?
Hi
I read these papers that maybe could help you:
Andersen, B., & Thrane, U. (1996). Differentiation of Altemaria infectoria and Alternaria alternata based on morphology, metabolite profiles, and cultural characteristics. Canadian Journal of Microbiology, 42(7), 685-689.
Thomma, B. P. (2003). Alternaria spp.: from general saprophyte to specific parasite. Molecular plant pathology, 4(4), 225-236.
Funnell-Harris, D. L., Prom, L. K., & Pedersen, J. F. (2012). Isolation and characterization of the grain mold fungi Cochliobolus and Alternaria spp. from sorghum using semiselective media and DNA sequence analyses. Canadian journal of microbiology, 59(2), 87-96.
Good luck
Hi, you can use either PDA or Malt Agar medium. You can also add streptomycin, to get rid of some bacteria etc, especially if you want to isolate Alternaria spp. from plant leaves. I found that sporulation work best with PDA and no streptomycin. You can also put your fungi under blacklight (2 weeks is a good start), which help a lot to obtain conidias. The color of the culture depends on the species, for example A. dauci produces some red pigments, A brassicicola or alternata produce black pigments...
Have a nice day
my suggestion is to use PDA with antibiotic ampicillin for 7days at 25oC.Alternate dark and light period also influence on sporulation of fungi
I generally isolate Alternaria sp. during the isolation of other fungi, from the soil ground and from different plant portions. In my opinion Alternaria sp. can be isolated very well on PDA medium with streptomycin, in 3-5 days at 23 °C. To obtain conidia, Alternaria sp. can be left to the growth in the dark for about 10 days, during wich the colony is observed at the optical microscope to check the conidia development.
Hi: PDA medium is the best but if you have problem with sporulation, some species of Alternaria is hard to sporulate on PDA you can use V8 medium.
During my investigation i had a lot problem with sporulation. One way is leave Petri dishes with Alternaria 7-days colonies on UV light for circa about 12 hours.
Pusz W. 2009. Morpho-physiological and molecular analyses of Alternaria alternata isolated from seeds of Amaranthus. Phytopathologia 54: 5-14
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