Md. Ekramul Karim

10 Questions 7 Answers 0 Followers

Questions related from Md. Ekramul Karim

How to determine the total phosphate accumulated in bacterial cell?

I'd like to know the determination of total phosphate accumulated in bacterial mass.

11 April 2018 6,265 2 View

Is it mandatory to construct standard curve of a specific sample during each spectrophotometric measurement?

For spectrophotometric analysis, could I use the standard curve for a specific solution every time, once it is constructrd? Is it necessary to construct the curve every time before starting of...

12 December 2016 8,564 3 View

What is the detection limit of hexavalent chromium by Diphenylcarbazide method?

During DPC method I got a problem. After adding DPC reagent into the sample under study, the characteristics purple color developed but soon disappears within seconds. Hence, i would like to know...

24 November 2016 1,060 1 View

How to fit a standard curve for Cr (vi) reduction assay by DPC method?

I'm trying to fit a standard curve for Cr (vi) assay by DPC method. But my curve didn't fit well (R-squared value 0.85). Would you please provide any suggestions for the best fit of the curve.?...

30 October 2016 7,157 1 View

Which primer / primer pairs could I use to amplify bacterial azoreductase gene and bacterial chromate reductase gene?

I would like to detect and amplify bacterial azoreductase gene, responsible for azo dye degradation and bacterial chromate reductase gene, responsible for Cr (VI) reduction. I would like to know...

04 August 2016 9,445 1 View

Why isotherm model such as Langmuir-Freundlich isotherm, is applied for biosorption by live/dead biomass? How do I construct this type of isotherm?

Thanks for your feedback

09 May 2016 8,012 2 View

What are the suitable media for characterization of Alternaria spp.?

I'd like to isolate Alternaria from plant leaves. Which media is best for rapid fungal growth as well as sporulation ? Are there any pre-requisitics growth conditions in order to induce...

17 April 2016 3,803 8 View

What is the best way of quantitative measurement of bacterial nitrogen fixation?

I want to determine the nitrogen fixation efficiency of some bacterial isolates in my lab. How can i do it with simplest lab settings?

05 April 2016 8,268 4 View

Which kit and primers can be used for fungal DNA isolation and identification?

I want to isolate fungal DNA from culture plate (from mycelium). Which kit is suitable for this? Which primers should i use for downstrean processing , e.g., PCR and sequencing for phylogenetic...

24 March 2016 3,777 4 View

How can I determine EC50 value from MIC values of metal-bacterial interaction?

Actually, i have got the MIC of a heavy metal against tested bacteria in my lab. Now, I'm going to determine EC50 .I want to know the simplest way of determining EC50 from MIC value.

13 March 2016 8,155 6 View