I was wondering if anyone has tried storing 8M urea solutions at cold temperature and still been able to use them. Everything I read says to make the solution fresh. In the lab I work in we filter all our solutions, so having to make it fresh each time and filter it is time consuming and costly. I was curious to see if it could be prepared and stored at -20C or -80C and be stable for a few weeks. Has anyone tried this and found the solution to be stable?
Ice formation should probably break some urea molecules.
Try at ambient temperature: the diacetylmonoxim-thiosemicarbazid method is easy for the determination of urea concentration (after dilution), then for validation of zero-hydrolysis.
Better to prepare fresh solution always. Its annoying but its better :)
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