We are working on the development of diagnostic systems for cystic fibrosis. We are having problems in the storage of our IRT proteins. The protein is degraded upon freeze thawing. Storage in PBS seems to disrupt the structure. We tried storage at pH:4.8. It works better, but we are still not sure what is the best method for storage. Any suggestion will help while we are pursuing our optimization work.
Hello
Try with ( pH on 6.8 to 8.9 ) .
In general the method of choice depends completely on the stability of the protein and on when and how you want to use it later on.
Good Luck
http://www.ibl-international.com/media/catalog/product/R/E/RE68015_IFU_en_IRT_Neonatal_LUM_2015-04-29_sym3.pdf
http://www.ibl-international.com/media/catalog/product/f/i/file_35.pdf
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3227416/
http://www.clinchem.org/content/56/3/445.full.pdf
http://www.clinchem.org/content/44/10/2094.full
Article Immunoreactive Trypsinogen (IRT) as a Biomarker for Cystic F...
- Badges
- Science method