What are the steps in measuring algal growth curve using spectrophotometer ?

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Why does exogenously applied iron salts cause leaves to become greener under sufficient iron conditions?

Do iron salts increase photosynthesis, or do they increase the size of chloroplasts, or cytochromes when iron is sufficient? In other words, is greening-up functional or structural under these...

26 January 2021 9,584 4 View

Why do my fibroblasts under hypoxia conditions proliferate slower than the same fibroblasts under normoxic conditions?

I am using healthy control lung fibroblasts in a 96 hour experiment with two drugs (nintedanib and Pirfenidone) and vehicle controls for both. I have both hypoxia (approx 1% O2) and normoxia...

12 January 2021 6,260 1 View

Mitigating future academic stressors during pedagogical development and instructional design?

Greetings from Vancouver, Canada! My doctoral research is going to begin soon. Because the instance of higher education students in Canada, and elsewhere, has been on the rise for more than a...

11 November 2020 2,600 3 View

How do you calculate the substrate concentration of an assay in enyzme kinetics given the volume and the buffer volume at a given 6mM of the enyzme?

In an attempt to plot the michaelis- menten curve, it requires the initial rate as the y-axis and the substrate contration as the x-axis. Now how do you get the substrate concentration of the...

25 October 2020 1,702 3 View

How to crystallize curcumin from ethanolic turmeric extract?

I'm having difficulty recovering curcumin from my ethanolic extract. I tried reducing its solubility in the solution by slowly adding water, but the solid just crashed out of the solution in very...

03 September 2020 3,020 5 View

Trace metal speciation?

I am carrying out a research to assess the ecological risk associated with the speciation of trace and toxic metals in floodplain suspension. Bearing in mind that the reducible, oxidizable and...

18 August 2020 4,333 4 View

What technique can be used for imaging wet hydrogels?

I'm working on alginate-based hydrogels and I want to observe the microstructures (porosity, roughness, etc.). The hydrogels were produced by gelation in aqueous calcium chloride. SEM is out of...

30 August 2019 2,863 4 View

What is the best way to hold AFM cantilevers during piranha cleaning?

Hi, I'm trying to use piranha to clean cantilevers (silicon and gold coated SiNi) but I find that bubbles form on the cantilever chips and cause them to start to float or flip over, potentially...

24 March 2019 8,787 1 View

Is rice bran oil good for frying?

I am considering rice bran oil for frying to replace canola oil due to its benefits. I noticed difference in the consistency of the food, in this case the quality of caramel and its adhesion to...

29 May 2018 1,311 1 View

Can somebody assist me by giving me standards for fumonisins for my MSC project?

I am a Master of Biotechnology student at Kenyatta University, working on a project on determination of Fumonisin FB1, FB1, FB3 and FB4 on maize samples. I would like to use HPLC technique of...

30 October 2017 2,079 3 View

How to normalise data on OPM package from R Studio ?

Good afternoon, I recently used OmniLog from BIOLOG for my experimentations : I tested the metabolism of different strains on 2 types of plates. I have 16 strains of 3 different groups...

02 March 2021 3,584 1 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Any research papers for extraction of nutrient deprivation on macroalgae? Analysed by HPLC-UV and GC/MS. Possibly from macroalgae genus Rhizoclonium?

I am doing my thesis on the nutrient deprivation on the macroalgae Rhizoclonium sp. I will be analysing the levels over a period of roughly 5 days by HPLC-UV and GC/MS. I will be using seawater as...

28 February 2021 6,477 1 View

Has anyone used the Illumina ITS1 primer sets?

Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...

25 February 2021 6,969 3 View

Unknown debris sheets observed in cell culture flasks?

Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...

25 February 2021 3,443 4 View

Is there any one who can provide a donation of HPV detection and genotyping kits in exchange collaboration fors?

Warm greetings My name is Ayichew S. and I am doing my Ph.D. in Medical Microbiology (on Human papillomavirus). At this stage, I have almost completed a clinical sample Cervical swabs)...

25 February 2021 1,660 3 View

Is there any one who can provide a donation of HPV detection and genotyping kits in exchange collaboration forms?

Warm greetings! My name is Ayichew S. and I am doing my Ph.D. in Medical Microbiology focusing on Human papillomavirus). At this stage, I have almost completed a clinical sample (Cervical swabs)...

25 February 2021 3,612 3 View

Advice on growing lactobacillus species in the lab?

Hello everyone, For part of my PhD project, I'm hoping to grow some lactobacillus species in the lab so that I can create standard curves for a qPCR quantification of a faecal sample. However, I...

24 February 2021 3,157 6 View