Which could be the best solvent for animal secretions in GCMS?

While doing experiments with snail slime/mucous, it is difficult to find the best suitable solvent for extraction. Please suggest one best suitable solvent for extraction for GCMS procedures of...

03 April 2018 4,182 0 View

Which is the best way to visualize microsatellite bands?

Which is the best way to visualize microsatellite bands which are ranging from 100 bp to 300 bp? while loading the PCR product on agarose gel with 1.5% concentration very faint and low quality...

07 August 2017 6,171 15 View

Smearing of DNA bands on gel.

When I was doing PCR amplification of a cyt b gene of 1200bp in size and was getting positive bands of about 1000 bp on agarose gel at an annealing temperature of 53 degree celsius. But later when...

07 August 2017 834 3 View

What are the bands in my gel?

After many trial and errors I got a band in my PCR amplification. I have used taq, DNTP mix from the same company (Termo scientific). I think it could have helped me. But now the problem is the...

04 May 2016 9,827 8 View

What is the reason for Primer Dimer formation in a PCR reaction? What is the role of PCR reagents in Primer Dimer formation?

Again while doing the PCR amplification of snail DNA, I got stuck with no visible bands on gel. But there is the presence of primer dimers in the gel. The DNA pellet I got readily dissolved in...

01 February 2016 5,284 5 View

What are the bands in the gel image?

while isolating DNA from animal tissue I usually get bands in the upper region of the gel. But today after DNA isolation AGE was done at 100v for 30 minutes and the gel documentation yielded the...

01 February 2016 4,801 5 View

Can I use a DNA sample with a nanodrop value (260/280) 1.11 for PCR amplification?

While doing the PCR amplification of 16s rRNA gene of snail samples, I got a very nice pellet of DNA which dissolved in water readily. But when the PCR amplification was performed there appeared...

31 December 2015 2,420 6 View

What is GoTaq  Flexi buffer, GoTaq  Flexi (Promega) and labeled universal tail. in microsatellite PCR reaction? Is it availabe in India?

While doing the micro satellite analysis, on reading an article, I came to stuk with these terms. I became bit confused abot the availability of these things in India. Can anyone help me out with...

06 July 2015 4,796 0 View

How can I remove the contents of methanol from a methanol extracted plant sample?

We have done methanol extraction of a plant sample using a soxhlet apparatus. In order to get rid of the contents of methanol from the extract what procedure has to do? Simply evaporating the...

10 November 2014 847 5 View

How do you design primers for ISSR in snails?

I am working on the molecular phylogeography of giant african snail in S. India. I would like to know how can I design the primers for the ISSR regions of the snail? Can I use a general primer...

08 September 2014 3,716 3 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

B-mercaptoethanol (BME) protocol?

Hi everybody, I have been running a multiplex IHC which allow me to stain the same tissue with different markers (Primary Ab + Secondary conjugated Ab) (AF487, AF555, AF647). Since I would like to...

27 February 2021 5,028 2 View

Why do my MCFs not form monolayer?

Hello, I am looking for some expertise on culturing MCF7s. I purchased them from ATCC and I have been following all media and culture conditions. Media is EMEM with 10% FBS and 10ug/mL of...

27 February 2021 3,904 2 View

Is there anyone who experienced with cytotoxic effect of Hoescht 33342 dye?

Is Hoechst toxic to cells? In general, it is known as non-toxic. I am working with dsRED MDA-MB-231 cell line. I applied it for my PDMS-based microfluidic platform, for this reason, I incubated...

26 February 2021 9,119 3 View

Why my agarose gel staining sometimes looks not homogenic but like gradient (see the figure)?

I am worried about this overexposure of the upper part of the gel in the picture. Is it possible that this is the result of too much concentration of ethidium bromide? Why is there such a big...

25 February 2021 8,140 3 View

Markers of mitochondrial activity in FFPE tissues?

Hello everybody, Does someone have any experience with assessing/extimating mitochondrial activity on FFPE tissues? Precisely, no TOM20, ATP5B, etc: we are trying to get an approximative idea of...

24 February 2021 4,618 4 View

Can a Golgi-stained dendritic spines picture processed by Image J be directly used for publication?

24 February 2021 3,348 2 View

Is there an appropriate CD8 antibody for IHC-P for staining human breast cancer tissues?

Hello, We are planning on staining Formalin-fixed paraffin breast cancer tissue sections (FFPE) with CD8 Ab via immunofluorescence (Tissue IF). Based on our existing protocol and imaging...

24 February 2021 9,578 4 View

Reagents for histology slides?

Dear all, I am planning to study histology and want to do that at home. I have only stained a blood smear with Giemsa but now want to try a wider range of animal tissues. What reagents will I...

24 February 2021 5,430 3 View

Are there lab markers/pens resistant to Roti-Histol?

Hi, I'm looking for a pen/marker to write on the smooth, transparent (non-frosted) part of microscopy slides which does not get washed away by alcohols and most importantly Roti-Histol. I want to...

24 February 2021 9,902 5 View