Since the Geimsa stains are very costly for doing karyotyping, whichare the other cheap stains which can be used for karyotyping?
31 December 2019 8,430 3 View
While doing experiments with snail slime/mucous, it is difficult to find the best suitable solvent for extraction. Please suggest one best suitable solvent for extraction for GCMS procedures of...
03 April 2018 4,182 0 View
Which is the best way to visualize microsatellite bands which are ranging from 100 bp to 300 bp? while loading the PCR product on agarose gel with 1.5% concentration very faint and low quality...
07 August 2017 6,171 15 View
When I was doing PCR amplification of a cyt b gene of 1200bp in size and was getting positive bands of about 1000 bp on agarose gel at an annealing temperature of 53 degree celsius. But later when...
07 August 2017 834 3 View
After many trial and errors I got a band in my PCR amplification. I have used taq, DNTP mix from the same company (Termo scientific). I think it could have helped me. But now the problem is the...
04 May 2016 9,827 8 View
Again while doing the PCR amplification of snail DNA, I got stuck with no visible bands on gel. But there is the presence of primer dimers in the gel. The DNA pellet I got readily dissolved in...
01 February 2016 5,284 5 View
while isolating DNA from animal tissue I usually get bands in the upper region of the gel. But today after DNA isolation AGE was done at 100v for 30 minutes and the gel documentation yielded the...
01 February 2016 4,801 5 View
While doing the PCR amplification of 16s rRNA gene of snail samples, I got a very nice pellet of DNA which dissolved in water readily. But when the PCR amplification was performed there appeared...
31 December 2015 2,420 6 View
We have done methanol extraction of a plant sample using a soxhlet apparatus. In order to get rid of the contents of methanol from the extract what procedure has to do? Simply evaporating the...
10 November 2014 847 5 View
I am working on the molecular phylogeography of giant african snail in S. India. I would like to know how can I design the primers for the ISSR regions of the snail? Can I use a general primer...
08 September 2014 3,716 3 View
Hello! I want to quantify by ELISA the secreted (from platelets poor plasma) and the non-secreted (from platelet lysate) PF4 before and after TRAP stimulation. I will use the ELISA from R&D...
03 March 2021 1,499 2 View
Hello, If i am doing a buffer exchange for an antibody of 1mg/mL, does the elution lose protein in the process of buffer exchange? For example, if i flow through 500 uL of 1mg/mL sample, and...
03 March 2021 6,299 3 View
Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...
02 March 2021 4,029 3 View
Hello! I'll do a size exclusion chromatography, but I only have an open column, and I'll perform the peptide extraction from yeast, using buffer lysis (sodium phosphate 50 mM/NaCl 30 mM/DNAse and...
01 March 2021 2,215 2 View
I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...
01 March 2021 8,544 1 View
Can someone please give me some possible things that could go wrong? Here is my recipe: 0.5g Agarose 50 mL of TAE 1x 1 uL ethyl bromide. Gel was run at 100V for 1 hour. The buffer used is also TAE.
01 March 2021 9,952 3 View
To dear Researchers, I was analyzing a series of concentration for estimation of Real-Time PCR efficiency. The concentration was 1:10. I used MS-excel to evaluate Slope. The result of slope was -8...
01 March 2021 8,683 4 View
Does anyone have the experience of using Taq Man probes in the QIAGEN Rotar- Gene qPCR machine?
01 March 2021 5,311 1 View
I'm looking at the aggregation of my protein sample using DLS. Unfortunately, my buffer (20mM HEPES) also results in a set of peaks. These are at approximately 1 and 1000 d.nm. The lowest peak...
01 March 2021 9,015 2 View
Dear All, mirna primer showing some problem in the melting curve? any idea why? As attached is the melting curve. The forward sequence is obtained from miRBase and reverse primer is universal.
28 February 2021 5,008 4 View
