Keerthy Vijayan

11 Questions 8 Answers 0 Followers

Questions related from Keerthy Vijayan

What are the stains other than Geimsa stain used for karyotyping?

Since the Geimsa stains are very costly for doing karyotyping, whichare the other cheap stains which can be used for karyotyping?

01 January 2020 8,494 3 View

Which could be the best solvent for animal secretions in GCMS?

While doing experiments with snail slime/mucous, it is difficult to find the best suitable solvent for extraction. Please suggest one best suitable solvent for extraction for GCMS procedures of...

04 April 2018 4,232 0 View

Smearing of DNA bands on gel.

When I was doing PCR amplification of a cyt b gene of 1200bp in size and was getting positive bands of about 1000 bp on agarose gel at an annealing temperature of 53 degree celsius. But later when...

08 August 2017 891 3 View

Which is the best way to visualize microsatellite bands?

Which is the best way to visualize microsatellite bands which are ranging from 100 bp to 300 bp? while loading the PCR product on agarose gel with 1.5% concentration very faint and low quality...

08 August 2017 6,221 15 View

What are the bands in my gel?

After many trial and errors I got a band in my PCR amplification. I have used taq, DNTP mix from the same company (Termo scientific). I think it could have helped me. But now the problem is the...

05 May 2016 9,881 8 View

What is the reason for Primer Dimer formation in a PCR reaction? What is the role of PCR reagents in Primer Dimer formation?

Again while doing the PCR amplification of snail DNA, I got stuck with no visible bands on gel. But there is the presence of primer dimers in the gel. The DNA pellet I got readily dissolved in...

02 February 2016 5,346 5 View

What are the bands in the gel image?

while isolating DNA from animal tissue I usually get bands in the upper region of the gel. But today after DNA isolation AGE was done at 100v for 30 minutes and the gel documentation yielded the...

02 February 2016 4,850 5 View

Can I use a DNA sample with a nanodrop value (260/280) 1.11 for PCR amplification?

While doing the PCR amplification of 16s rRNA gene of snail samples, I got a very nice pellet of DNA which dissolved in water readily. But when the PCR amplification was performed there appeared...

01 January 2016 2,479 6 View

What is GoTaq Flexi buffer, GoTaq Flexi (Promega) and labeled universal tail. in microsatellite PCR reaction? Is it availabe in India?

While doing the micro satellite analysis, on reading an article, I came to stuk with these terms. I became bit confused abot the availability of these things in India. Can anyone help me out with...

07 July 2015 4,849 0 View

How can I remove the contents of methanol from a methanol extracted plant sample?

We have done methanol extraction of a plant sample using a soxhlet apparatus. In order to get rid of the contents of methanol from the extract what procedure has to do? Simply evaporating the...

11 November 2014 938 5 View

How do you design primers for ISSR in snails?

I am working on the molecular phylogeography of giant african snail in S. India. I would like to know how can I design the primers for the ISSR regions of the snail? Can I use a general primer...

09 September 2014 3,771 3 View