While doing experiments with snail slime/mucous, it is difficult to find the best suitable solvent for extraction. Please suggest one best suitable solvent for extraction for GCMS procedures of snail slime.
Since the Geimsa stains are very costly for doing karyotyping, whichare the other cheap stains which can be used for karyotyping?
31 December 2019 8,539 3 View
When I was doing PCR amplification of a cyt b gene of 1200bp in size and was getting positive bands of about 1000 bp on agarose gel at an annealing temperature of 53 degree celsius. But later when...
07 August 2017 956 3 View
Which is the best way to visualize microsatellite bands which are ranging from 100 bp to 300 bp? while loading the PCR product on agarose gel with 1.5% concentration very faint and low quality...
07 August 2017 6,276 15 View
After many trial and errors I got a band in my PCR amplification. I have used taq, DNTP mix from the same company (Termo scientific). I think it could have helped me. But now the problem is the...
04 May 2016 9,943 8 View
while isolating DNA from animal tissue I usually get bands in the upper region of the gel. But today after DNA isolation AGE was done at 100v for 30 minutes and the gel documentation yielded the...
01 February 2016 4,889 5 View
Again while doing the PCR amplification of snail DNA, I got stuck with no visible bands on gel. But there is the presence of primer dimers in the gel. The DNA pellet I got readily dissolved in...
01 February 2016 5,400 5 View
While doing the PCR amplification of 16s rRNA gene of snail samples, I got a very nice pellet of DNA which dissolved in water readily. But when the PCR amplification was performed there appeared...
31 December 2015 2,545 6 View
While doing the micro satellite analysis, on reading an article, I came to stuk with these terms. I became bit confused abot the availability of these things in India. Can anyone help me out with...
06 July 2015 4,900 0 View
We have done methanol extraction of a plant sample using a soxhlet apparatus. In order to get rid of the contents of methanol from the extract what procedure has to do? Simply evaporating the...
10 November 2014 1,023 5 View
I am working on the molecular phylogeography of giant african snail in S. India. I would like to know how can I design the primers for the ISSR regions of the snail? Can I use a general primer...
08 September 2014 3,822 3 View
Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...
11 August 2024 7,082 3 View
I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...
08 August 2024 9,882 2 View
I need to do some experiment with it in liquid form.
07 August 2024 613 3 View
I am having an issue with my gel image where my PCR product is not appearing very bright on the gel. When I perform gel extraction, the A260/280 purity value is very low. I used the Qiagen gel...
05 August 2024 9,798 3 View
I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...
04 August 2024 501 2 View
HOW CAN I WRITE A CODE TO USE THE WAVENET TRANSFORM AS A FEATURE EXTRACTION METHOD INSTEAD OF DWT IN MATLAB?
03 August 2024 7,829 0 View
I work on MCF7 cell cell for anticaner purpose and I wa to do drug preperation the drug ( secondary metabolites extracted from Aspergillus) My question which solvent is better with these secodary...
03 August 2024 4,725 2 View
Hello everyone, I need to dissolve stigmasterol for an animal experiment, but it hasn't dissolved in either DMSO or vegetable oil. Can anyone recommend the best solvent for stigmasterol?
01 August 2024 7,504 2 View
I am currently working on a project that involves extracting cell membranes, for which we disrupt the cells using sonication. During the initial extraction process, we add protease inhibitors to...
30 July 2024 7,077 1 View
I am cloning an overexpression plasmid with my protein of interest tagged with mScarlet. After transforming my ligated product into DH5α bacteria and plating on LB agar, I noticed colonies with a...
22 July 2024 5,953 6 View