I remember to have read about the side effects of ablation of Nestin in adolescent mice - not in embryos (e.g. in a model with Nestin/Cre-ERT2/diphtheria toxin model): tremor, shivering and ataxia set in after a while - I would need to know more about those side effects (at what timepoint after ablation they set in? after how many injections of tamoxifen?). 

Do you have experience with those side effects? Can you share any literature on it? 

Thank you very much!