hello I hope you have a good day, I am working on the expression of genes p5cr, p5cdh, prot1, prot2, and aap6 of corn plants
What primer should I use?
thanks for your guide
Start by looking for publications that measure expression of these genes. If there aren't any, then you'll have to design them yourself.
Note - just because it's published doesn't mean it's the best ones to use. Set up controls & test them yourself!
Good luck!
Hello, if possible, please send me new methods of management and communication in the classroom. thank you؟
18 June 2024 4,139 3 View
A magnetic nanocomposite has been synthesized and its electrical conductivity needs to be measured. What analysis and technique can be used to measure the electrical conductivity of this magnetic...
16 June 2024 7,488 3 View
We know that one of the important members of a 3D Geomechanical Model is in-situ stresses' directions and magnitudes. we can use the seismic data to estimate these parameters in special record...
21 May 2024 1,332 2 View
surface tension
20 May 2024 4,895 1 View
what are the S-scheme and Z-scheme heterojunctions in photocatalytic systems? and for the types of heterogeneous photocatalysts, how can you determine the type of S-scheme and Z-scheme?
16 May 2024 1,545 1 View
Hello everyone, I hope you are all doing well. I have already checked plagiarism, related articles’ structures, and grammar, and they seem fine, but good impact factor journals that I have written...
15 May 2024 1,007 4 View
hi there i'm working on quadrotor path tracking in matlab's simulink . there is an inner loop which is responsible for tracking the desired euler angles generated by the outer loop . feedback...
05 May 2024 7,705 5 View
Hello Dears, I am currently working on time-dependent density functional theory (TDDFT) calculations for both singlet and triplet states using the LC-BLYP functional in GAMESS. I believe I have...
01 May 2024 4,614 0 View
greetings, I'm culturing SW480 cells on DMEM high glucose gibco with FBS. When I'm performing MTT Assay, upon treatment of cells with Baicalein, the color of medium with 5% FBS changes to dark...
25 April 2024 7,115 4 View
hi there . i'm currently working on a thesis which involves designing a fast nonsingular terminal sliding-mode controller for attitude control of a quadrotor. the outputs of sliding mode...
20 April 2024 425 2 View
After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...
08 August 2024 1,668 3 View
I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...
05 August 2024 9,059 3 View
How to design VN primer to attach with universal reverse primer
05 August 2024 2,116 3 View
It's an end-point PCR protocol. I'm using 1.5% agarose gel with SyBR Safe dye and TBE as a running buffer, visualization on BioRad XR+ system. I was primarily thinking of primer efficiency,...
01 August 2024 4,673 4 View
Hello everyone, I performed a PCR yesterday, and the results showed no bands on the gel. Of course, I probably missed some crucial steps, like adding my samples to the PCR strips themselves, for...
31 July 2024 2,406 6 View
Hello all, I have been trying to follow a 2-stage PCR protocol used to amplify barcodes of a large yeast library, as per Nyugen et al. (2022) -...
30 July 2024 841 2 View
I want to introduce 2 mutations using Agilent's multi-site mutagenesis kit, I designed the primers using their online tool and it gave me 2 sets for primers (1 set for each mutation) so I was...
25 July 2024 6,517 3 View
Hi everyone, I have extracted DNA from a biogas bioreactor using Qiagen kit and prep cDNA library then used this library as template to optimize primers for qPCR (taken from papers). Some of the...
23 July 2024 1,329 5 View
Hello researchers, Sorry for my stupid question. I am learning the QIIME2 workflow for analyzing some 16s amplicon NGS fastq data. I found a very nice paper with data and code public available...
20 July 2024 5,405 2 View
We are in need of primers for a PCR protocol for chicken genome. We are looking at the COI barcode, 648-bp fragment of the 5' end of COI. Specifically Primers FalcFA, BirdR1, CO1_ExtF,...
16 July 2024 118 2 View