What best method could be used to screen protein expression profile in a cancer cell line upon novel drug testing?

I am studying gene expression profile through analyses of proteins expressed upon treatment of cancer stem cell line with a novel herbal substance (in the extract) to predict gene function in...

07 August 2019 5,634 7 View

Unbearable article publication charges is the main reason for proliferative number of publications in the predatory journals. Yes or No?. Reasons?

There are many factors to think about as possible reasons that give rise to proliferative number of publications in predatory journals. Arguably, article publication charges has been pointed as...

06 July 2019 833 24 View

What should you do when your journal send you a huge payment list after publication, that was not pre-informed before submission?

I want to know people's opinions and how to handle a case of one's journal that had already published their article, indexed it in popular databases, but as a result of their changing publisher...

05 June 2019 6,811 3 View

Is Impact Factor (IF) truly the best metric index in judging the quality of a journal?

So many metrics are currently in-use by various journals to show the quality of their journals. Again, the seemingly most mentioned metric s, Impact Factor (IF) is largely criticized as biased and...

04 May 2019 8,222 15 View

What best advice could you offer a group of authors whose article has been accepted and issue-listed but not published for more than 7 months?

How do you advise a group of authors whose papers already accepted but only listed for several months under ahead-of-print without publication as necessary?

04 May 2019 3,104 6 View

What's the meaning of each of the following terms- Cited Half-Life, Immediacy Index, Eigenfactor, Article Influence used by RG for journal ratings?

These terms (Cited half-life, Immediacy Index, Eigenfactor, Article Influence) are used by Researchgate to measure the impact of any article uploaded on their system. They are also used to rate...

03 April 2019 2,144 3 View

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07 August 2018 383 2 View

What is the difference between these two acronyms, ESCI and SCIE indexes in considering better journal publisher?

v Which of these two indexes really has affiliation with ISI, between Science Citation Index Expanded (SCIE) and Emerging Sources Citation Index (ESCI)? v And what does each of them stand for? v...

06 July 2018 7,195 1 View

How and where do you get ethical approval in this case scenerio?

You observe some dietary habits in humans living in your vicinity and willing to find out if this will be causal for suspected deficiencies in some blood biomarkers. Fine, they are willing to...

03 April 2018 1,304 0 View

Standard deviation (SD) or standard error of mean (SEM): which way to go; what are the factors to consider in reporting?

Which one under- or overestimates significance in the test of association?. Often times, I come across both choices in the selection of SD and SEM as a measure of spread, however, little is...

01 February 2018 4,122 2 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

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02 March 2021 3,060 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Which fluorescent dye to use for measuring total ROS production?

Is DCFDA appropriate for measuring total ROS production, or can it only detect certain types of ROS? If I want to measure total ROS production, which dye should I use?

01 March 2021 9,447 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View