What are the possible reasons why TEA is not blocking inactivation of outward currents of plant protoplasts?

05 May 2015 1 10K Report

What are the possible reasons why the TEA is not blocking inactivation outward currents of plant protoplast, I got good blocking currents with Bacl2?

Anton R Schäffner

Hi Rajesh,

first I want to clarify your question, since it contains two negative terms, so is your observation "positive", i.e. (i) not blocking inactivation means that you have an inactivation by whatever means and this is not overcome by TEA or (ii) I assume that you mean was why TEA does not block outward currents.

My simple answer to (ii) would be that TEA is applied outside, so it would not interfere with the outward transport of K+.

Best wishes,

Tony Schaeffner

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