Hi Rajesh, you data seem to fit well with a single exponential rise to max equation - you may want to compare the time constant of recovery and maximal recovery (both values are given by the fit). The fact you don't get full recovery at 5 s is not an issue by itself - the question is rather whether you would get full recovery after longer period of time as you may possibly have a low recovery competent.

Thanks Norber Weiss,

Can you share your plot, which software you used?

Best,

Rajesh Vinnakota

Here it is. It is fitted with an equation called "exponential association" in prism:

Y=Y0 + (Plateau-Y0)*(1-exp(-K*x))

Time constant: 0.972 vs 0.944

Plateau (i.e. recovered fraction): 84.0 vs 96.2

And arguably 100-plateau gives you the non-recovered fraction

This is done on the average curves but if you do it on each individual recordings then you can run statistics and see whether these differences are significant.

Hope it helps.

Thanks Norber Weiss. Hope your suggestion will helps me a lot. I don't have prism, and is it possible to with Orign?

Best regards,

Rajesh Vinnakota

Of cause Origin is perfectly fine!

Hi Norbet Weiss,

I am still not able to fit the above data in the Origin, Herewith I am sending the Origin screenshots which I tried to fit with "exponential association". If possible please go through it and let me know the way of fitting the curve with ""exponential association" equation.

Thanks for all your suggestions.

Best,

Rajesh VInnakota